Merkur'eva R V
J Hyg Epidemiol Microbiol Immunol. 1978;22(4):400-7.
Theoretical basis and results of biochemical studies of the enzymic systems of different localization in the cell--both bound with subcellular structures lysosomes--and dissolved in the cytosol--during experimental exposure to some environmental factors (carbon tetrachloride, carbon disulphide and others) are presented. It has been suggested to employ the assessment of the functional state of biomembranes of cellular organelles (e. g. lysosomes) and especially the effect of solubilization of membrane-bound enzymes and labilization of lysosomal membranes as one of the biochemical criteria of hygienic assessment of toxic effects and of some forms of delayed effects of environmental contaminants. Simultaneous biochemical investigation of enzyme-markers of subcellular organelles in the tissues of various organs and in biological fluids (blood, urine, seminal and amniotic fluids) permits us to elaborate and recommend for use in hygienic practice new biochemical criteria of unfavourable biological effects of chemical factors of the environment.
本文介绍了在实验性暴露于某些环境因素（四氯化碳、二硫化碳等）期间，对细胞中不同定位的酶系统进行生化研究的理论基础和结果，这些酶系统既与亚细胞结构溶酶体结合，又溶解于细胞质中。有人建议将细胞器（如溶酶体）生物膜功能状态的评估，特别是膜结合酶的溶解作用和溶酶体膜的不稳定作用作为卫生学评估毒性作用和环境污染物某些形式的迟发作用的生化标准之一。对各种器官组织和生物体液（血液、尿液、精液和羊水）中亚细胞器的酶标记物进行同步生化研究，使我们能够制定并推荐在卫生实践中使用新的生化标准，以评估环境化学因素的不利生物学效应。
