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Lethal and chromosomal effects of freezing, thawing, storage time, and x-irradiation on mammalian cells preserved at -196 degrees in dimethyl sulfoxide.

作者信息

Ashwood-Smith M J, Friedmann G B

出版信息

Cryobiology. 1979 Apr;16(2):132-40. doi: 10.1016/0011-2240(79)90023-3.

DOI:10.1016/0011-2240(79)90023-3
PMID:573193
Abstract
摘要

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Lethal and chromosomal effects of freezing, thawing, storage time, and x-irradiation on mammalian cells preserved at -196 degrees in dimethyl sulfoxide.冷冻、解冻、储存时间及X射线辐射对在二甲基亚砜中于-196℃保存的哺乳动物细胞的致死及染色体效应
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Changes in cell size during the cooling, warming and post-thawing periods of the freeze-thaw cycle.
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Genetic damage is not produced by normal cryopreservation procedures involving either glycerol or dimethyl sulfoxide: a cautionary note, however, on possible effects of dimethyl sulfoxide.涉及甘油或二甲基亚砜的常规冷冻保存程序不会产生遗传损伤：然而，关于二甲基亚砜可能的影响需予以警示。
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[Complex influence of deep freezing (-79 degrees C) and glycerol or dimethyl sulfoxide on chromosomes of cells cultivated in vitro].
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Fixation of potentially lethal radiation damage in Chinese hamster cells by anisotonic solutions, polyamines, and DMSO.
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