University of Tuscia, Viterbo, Italy.
Mutat Res. 2010 Sep 10;691(1-2):23-6. doi: 10.1016/j.mrfmmm.2010.06.008. Epub 2010 Jun 23.
Ultrasoft X-rays have been shown to be very efficient in inducing chromosomal aberrations in mammalian cells. The present study was aimed to evaluate the modifying effects of DMSO (a potent scavenger of free radicals) on the frequencies of chromosome aberrations induced by soft X-rays. Confluent held G1 Chinese hamster cells (V79) were irradiated with Carbon K ultrasoft X-rays in the presence and absence of 1M DMSO and frequencies of chromosome aberrations in the first division cells were determined. DMSO reduced the frequencies of exchange types of aberrations (dicentrics and centric rings) by a factor of 2.1-3.5. The results indicate that free radicals induced by ultrasoft X-rays contribute to a great extent to the induction of chromosome aberrations. The possible implications of these results in interpreting the mechanisms involved in the high efficiency of ultrasoft X-rays in the induction of chromosome aberrations are discussed.
超软 X 射线已被证明在诱导哺乳动物细胞染色体畸变方面非常有效。本研究旨在评估 DMSO（一种有效的自由基清除剂）对软 X 射线诱导染色体畸变频率的修饰作用。用 1M DMSO 存在和不存在的情况下，用碳素 K 超软 X 射线照射汇合期 G1 中国仓鼠细胞（V79），并测定第一次分裂细胞中的染色体畸变频率。DMSO 将交换型畸变（双着丝粒和中央环）的频率降低了 2.1-3.5 倍。结果表明，超软 X 射线诱导的自由基在很大程度上导致了染色体畸变的诱导。这些结果在解释超软 X 射线诱导染色体畸变高效性所涉及的机制方面的可能意义进行了讨论。
