Falkowski J
Zentralbl Bakteriol B. 1979 May;168(3-4):361-6.
Thirty strains of the species Thermoactinomyces vulgaris (Tsiklinsky, 1988) were isolated from milk and tested for their lipolythic activity. After incubation for 120 hours at 55 degrees C they formed distinct diffusion zones on culture media containing tributyrin and tween-80. In all strains exocellular lipases were found. Their action on the milk fat increased the number of the free fatty acids. Triglycerides, free fatty acids as well as diglycerides and monoglycerides were identified as split products chromatographically. The activity of the endolipases was lower. In four of the most active endolipases cholesterol was identified as split product of milk fat in addition to those mentioned above.
从牛奶中分离出30株普通嗜热放线菌(齐克林斯基,1988年),并对其脂肪分解活性进行了测试。在55℃下培养120小时后,它们在含有三丁酸甘油酯和吐温80的培养基上形成了明显的扩散区。在所有菌株中均发现了胞外脂肪酶。它们对乳脂肪的作用增加了游离脂肪酸的数量。通过色谱法鉴定出甘油三酯、游离脂肪酸以及甘油二酯和甘油单酯为分解产物。内脂肪酶的活性较低。在四种活性最高的内脂肪酶中,除上述分解产物外,还鉴定出胆固醇是乳脂肪的分解产物。