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从甲醛琼脂糖凝胶转移至重氮苄氧基甲基纸上的前胶原信使核糖核酸的鉴定

Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels.

作者信息

Rave N, Crkvenjakov R, Boedtker H

出版信息

Nucleic Acids Res. 1979 Aug 10;6(11):3559-67. doi: 10.1093/nar/6.11.3559.

DOI:10.1093/nar/6.11.3559
PMID:573889
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327956/
Abstract

Poly A containing RNA isolated from embryonic chick calvaria was transferred from 6% formaldehyde 0.75% agarose gels to diazobenzyloxymethyl paper and the paper then hybridized to either nick translated pro alpha 1 collagen cDNA clones, pCg1 or pCg54, or to the nick translated pro alpha 2 collagen cDNA clone, pCg45. From the mobilities of the bands hybridizing most strongly to each, pro alpha 2 collagen mRNA was shown to be slightly larger than pro alpha 1 mRNA; they are 5100 and 4900 nucleotides long respectively. pCg54 also hybridized weakly to two bands of lower mobility, corresponding to RNAs 6.4 and 5.6 kb long. Neither pCg54 nor pCg45 hybridized to type II procollagen mRNA in poly A containing RNA isolated from embryonic chick sterna.

摘要

从胚胎鸡颅骨分离得到的含多聚腺苷酸的RNA，从6%甲醛、0.75%琼脂糖凝胶转移至重氮苄氧基甲基纸上，然后该纸与缺口平移的原α1胶原蛋白cDNA克隆pCg1或pCg54，或与缺口平移的原α2胶原蛋白cDNA克隆pCg45进行杂交。根据与每个克隆杂交最强的条带迁移率，显示原α2胶原蛋白mRNA略大于原α1 mRNA；它们分别长5100和4900个核苷酸。pCg54也与两条迁移率较低的条带弱杂交，对应于长度为6.4和5.6 kb的RNA。在从胚胎鸡胸骨分离得到的含多聚腺苷酸的RNA中，pCg54和pCg45均未与II型前胶原蛋白mRNA杂交。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9cb/327956/f61266aba610/nar00452-0143-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9cb/327956/4e53ac5bbfbd/nar00452-0139-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9cb/327956/aaefc4a6b61a/nar00452-0140-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9cb/327956/a42a336f9f6b/nar00452-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9cb/327956/f61266aba610/nar00452-0143-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9cb/327956/4e53ac5bbfbd/nar00452-0139-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9cb/327956/aaefc4a6b61a/nar00452-0140-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9cb/327956/a42a336f9f6b/nar00452-0141-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9cb/327956/f61266aba610/nar00452-0143-a.jpg

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