Alwine J C, Kemp D J, Stark G R
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4. doi: 10.1073/pnas.74.12.5350.
We describe a technique for transferring electrophoretically separated bands of RNA from an agarose gel to paper strips. The RNA is coupled covalently to diazobenzyloxymethyl groups on the paper. After transfer and appropriate treatment of the paper to destroy remaining diazo groups, specific RNA bands can be detected by hybridization with 32P-labeled DNA probes followed by autoradiography. This procedure allows detection of specific RNA bands with high sensitivity and low background.
我们描述了一种将琼脂糖凝胶中电泳分离的RNA条带转移至纸条上的技术。RNA通过共价键与纸上的重氮苄氧基甲基基团相连。转移后，对纸条进行适当处理以破坏剩余的重氮基团，通过与32P标记的DNA探针杂交，然后进行放射自显影，即可检测到特定的RNA条带。该方法能够以高灵敏度和低背景检测特定的RNA条带。
