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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Knudsen J, Clark S, Dils R

Biochem J. 1976 Dec 15;160(3):683-91. doi: 10.1042/bj1600683.

An acyl-thioester hydrolase was isolated from the cytosol of lactating-rabbit mammary gland. The purified enzyme terminates fatty acid synthesis at medium-chain (C8:0-C12:0) acids when it is incubated with fatty acid synthetase and rate-limiting concentrations of malonyl-CoA. These acids are characteristic products of the lactating gland. 2. The mol.wt. of the enzyme is 29000+/-500 (mean+/-S.D. of three independent preparations), as estimated by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. 3. The enzyme also hydrolyses acyl-CoA esters of chain lengths C10:0-C16:0 when these are used as model substrates. The greatest activity was towards dodecanoyl-CoA, and the three preparations had specific activities of 305, 1130 and 2010 nmol of dodecanoyl-CoA hydrolysed/min per mg of protein when 56muM substrate was used. 4. The way in which this enzyme controls the synthesis of medium-chain fatty acids by fatty acid synthetase is briefly discussed.

从泌乳兔乳腺的胞质溶胶中分离出一种酰基硫酯水解酶。当该纯化酶与脂肪酸合成酶和限速浓度的丙二酰辅酶A一起孵育时，它会在中链（C8:0 - C12:0）脂肪酸处终止脂肪酸合成。这些脂肪酸是乳腺的特征性产物。2. 通过在十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳估计，该酶的分子量为29000±500（三次独立制备的平均值±标准差）。3. 当将链长为C10:0 - C16:0的酰基辅酶A酯用作模型底物时，该酶也能水解它们。对十二烷酰辅酶A的活性最高，当使用56μM底物时，三次制备的比活性分别为每毫克蛋白质每分钟水解305、1130和2010 nmol十二烷酰辅酶A。4. 简要讨论了这种酶控制脂肪酸合成酶合成中链脂肪酸的方式。

Knudsen J, Clark S, Dils R

Biochem J. 1976 Dec 15;160(3):683-91. doi: 10.1042/bj1600683.

An acyl-thioester hydrolase was isolated from the cytosol of lactating-rabbit mammary gland. The purified enzyme terminates fatty acid synthesis at medium-chain (C8:0-C12:0) acids when it is incubated with fatty acid synthetase and rate-limiting concentrations of malonyl-CoA. These acids are characteristic products of the lactating gland. 2. The mol.wt. of the enzyme is 29000+/-500 (mean+/-S.D. of three independent preparations), as estimated by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. 3. The enzyme also hydrolyses acyl-CoA esters of chain lengths C10:0-C16:0 when these are used as model substrates. The greatest activity was towards dodecanoyl-CoA, and the three preparations had specific activities of 305, 1130 and 2010 nmol of dodecanoyl-CoA hydrolysed/min per mg of protein when 56muM substrate was used. 4. The way in which this enzyme controls the synthesis of medium-chain fatty acids by fatty acid synthetase is briefly discussed.

从泌乳兔乳腺的胞质溶胶中分离出一种酰基硫酯水解酶。当该纯化酶与脂肪酸合成酶和限速浓度的丙二酰辅酶A一起孵育时，它会在中链（C8:0 - C12:0）脂肪酸处终止脂肪酸合成。这些脂肪酸是乳腺的特征性产物。2. 通过在十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳估计，该酶的分子量为29000±500（三次独立制备的平均值±标准差）。3. 当将链长为C10:0 - C16:0的酰基辅酶A酯用作模型底物时，该酶也能水解它们。对十二烷酰辅酶A的活性最高，当使用56μM底物时，三次制备的比活性分别为每毫克蛋白质每分钟水解305、1130和2010 nmol十二烷酰辅酶A。4. 简要讨论了这种酶控制脂肪酸合成酶合成中链脂肪酸的方式。