Marsh E N, Holloway D E
Department of Biochemistry, University of Cambridge, UK.
FEBS Lett. 1992 Sep 28;310(2):167-70. doi: 10.1016/0014-5793(92)81321-c.
The gene encoding component S, the small subunit, of glutamate mutase, an adenosylcobalamin (coenzyme B12)-dependent enzyme from Clostridium tetanomorphum has been cloned and its nucleotide sequence determined. The mutS gene encodes a protein of 137 amino acid residues, with M(r) 14,748. The deduced amino acid sequence showed homology with the C-terminal portion of adenosylcobalamin-dependent methylmalonyl-CoA mutase [1989, Biochem. J. 260, 345-352] and a region of cobalamin-dependent methionine synthase which has been shown to bind cobalamin [1989, J. Biol. Chem 264, 13888-13895].
破伤风梭状芽孢杆菌中依赖腺苷钴胺素（辅酶B12）的谷氨酸变位酶的小亚基成分S的编码基因已被克隆，并测定了其核苷酸序列。mutS基因编码一个由137个氨基酸残基组成的蛋白质，分子量为14,748。推导的氨基酸序列与依赖腺苷钴胺素的甲基丙二酰辅酶A变位酶的C末端部分[1989年，《生物化学杂志》260卷，345 - 352页]以及已证明能结合钴胺素的钴胺素依赖型甲硫氨酸合酶的一个区域[1989年，《生物化学杂志》264卷，13888 - 13895页]具有同源性。
