心肌肥厚消退过程中的溶酶体和中性水解酶活性
Lysosomal and neutral hydrolase activity during the regression of cardiac hypertrophy.
作者信息
Cutilletta A F, Reddy M K, Dowell R T, Zak R, Rabionowitz M
出版信息
Recent Adv Stud Cardiac Struct Metab. 1975;7:111-8.
Cardiac hypertrophy was produced in rats by constriction of the ascending aorta. Removal of the constricting band 10 days after operation resulted in rapid decline in left ventricular (LV) weight and total ventricular RNA. Activities of acid RNase and beta-glucuronidase were elevated 3 days after aortic constriction. Activities of cathepsin D and alkaline RNase were unchanges. Activities of cathepsin D and acid RNase were unchanged 1 and 3 days after removal of constricting band. Ca2+-activated, neutral protease (CAF) isolated from postmitochondrial muscle supernatant was partially purified and characterized. CAF specifically degrades alpha-actinin when incubated with isolated myofibriles in the presence of Ca2+.
通过结扎升主动脉在大鼠中诱导出心脏肥大。术后10天移除结扎带会导致左心室（LV）重量和心室总RNA迅速下降。主动脉结扎3天后，酸性核糖核酸酶和β-葡萄糖醛酸酶的活性升高。组织蛋白酶D和碱性核糖核酸酶的活性未发生变化。移除结扎带1天和3天后，组织蛋白酶D和酸性核糖核酸酶的活性未发生变化。从线粒体后肌上清液中分离出的Ca2+激活中性蛋白酶（CAF）得到了部分纯化和表征。当在Ca2+存在的情况下与分离出的肌原纤维一起孵育时，CAF会特异性降解α-辅肌动蛋白。
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