Amendt K, Czachurski J, Dembowsky K, Seller H
J Auton Nerv Syst. 1979 Oct;1(1):103-7. doi: 10.1016/0165-1838(79)90009-2.
The location of those neurones in the brain stem that project to the intermediolateral column (ILC) from which preganglionic sympathetic neurones have their origin was studied by the method of retrograde transport of horseradish peroxidase (HRP). In cats 30--50 nl of a 30% HRP solution was injected into the region of the ILC at T3 or L1 on one side. After a survival period of 72 h the lower brain stem from C1 to the inferior colliculi was sectioned and prepared for histological study under brightfield illumination. Neurones stained with exogenous HRP were found in three regions: (a) in the ipsilateral, dorsomedial part of the nucleus of the solitary tract (NTS) (43% of all labeled neurones), in the cranial part of the NTS, and also on the contralateral side (7%); (b) in the ventrolateral reticular formation beginning at the level of the obex up to 8 mm cranial to the obex (25% ipsilateral, 3% contralateral); and (c) in the ventral part of the raphe nuclei (postpyramidal and inferior central nucleus) from 2 to 9 mm cranial to the obex (22%).
采用辣根过氧化物酶（HRP）逆行运输法，研究了脑干中向中间外侧柱（ILC）投射的神经元的位置，节前交感神经元起源于该中间外侧柱。在猫身上，将30微升30%的HRP溶液注射到一侧T3或L1水平的中间外侧柱区域。在72小时的存活期后，将从C1到下丘的低位脑干切片，并在明场照明下进行组织学研究。在外源性HRP染色的神经元发现于三个区域：（a）在同侧孤束核（NTS）的背内侧部分（占所有标记神经元的43%），在孤束核的颅侧部分，以及对侧（7%）；（b）在从闩平面开始到闩平面上方8毫米的腹外侧网状结构（同侧25%，对侧3%）；（c）在闩平面上方2至9毫米的中缝核腹侧部分（锥体后核和中央下核）（22%）。
