体外初次免疫应答中的细胞相互作用：特定细胞簇的需求。
Cell interactions in the primary immune response in vitro: a requirement for specific cell clusters.
作者信息
Mosier D E
出版信息
J Exp Med. 1969 Feb 1;129(2):351-62. doi: 10.1084/jem.129.2.351.
Abstract
Mouse spleen cells were found to associate in cell clusters during the primary immune response to sheep erythrocytes in vitro. About 10% of the cell clusters had the following unique properties; (a) they contained most, if not all, antibody-forming cells, (b) they contained only cells forming antibody to one antigen when cell cultures were immunized with two antigens, (c) the cells in clusters reaggregated specifically after dispersion, and (d) the specific reaggregation of clusters appeared to be blocked by antibody to the antigen. The integrity of cell clusters was required for the proliferation of antibody-forming cells, and prevention of clustering by mechanical means or by excess antibody blocked the immune response. Antibody and antigenic determinants on the surfaces of cells probably provide the basis for interaction. The unique microenvironment of cell clusters was essential for the primary immune response in vitro.
摘要
在体外对绵羊红细胞的初次免疫应答过程中，发现小鼠脾细胞会形成细胞簇。约10%的细胞簇具有以下独特特性：(a) 它们包含了大部分（如果不是全部）抗体形成细胞；(b) 当细胞培养物用两种抗原免疫时，它们仅包含针对一种抗原形成抗体的细胞；(c) 簇中的细胞在分散后会特异性地重新聚集；(d) 簇的特异性重新聚集似乎被针对该抗原的抗体所阻断。抗体形成细胞的增殖需要细胞簇的完整性，通过机械手段或过量抗体阻止细胞簇形成会阻断免疫应答。细胞表面的抗体和抗原决定簇可能为相互作用提供了基础。细胞簇独特的微环境对体外初次免疫应答至关重要。
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2
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Proc Natl Acad Sci U S A. 1968 Oct;61(2):542-7. doi: 10.1073/pnas.61.2.542.
3
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J Exp Med. 1965 Aug 1;122(2):423-40. doi: 10.1084/jem.122.2.423.
4
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5
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6
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7
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