Edlumd P O, Paalzow L K
Acta Pharmacol Toxicol (Copenh). 1977 Jan;40(1):145-52. doi: 10.1111/j.1600-0773.1977.tb02062.x.
A highly sensitive gas-liquid chromatographic method with electron capture detection of clonidine in plasma is described. The alkaline samples (100-200mul) are extracted into cyclohexane-butanol (9:1), re-extracted into 0.1 N sulphuric acid, made alkaline and back-extracted into cyclohexane-butanol. The pentafluorobenzyl derivatives of clonidine and the internal standard 2,-(2,4-dichlorophenylamino)-2-imidazoline are then formed, and excess of reagent is removed by solvent extractions. The minimum detectable quantity (MDQ) of clonidine and the internal standard are 2.5.10(-16) mol/sec. (3.3 picogram) and 2.8.10(-16) mol/sec (5.9 picogram), respectively. The smallest amount of clonidine which can be determined in plasma samples with a precision of less than 20 per cent S.D. is 200 picogram. The time course of clonidine in plasma after intravenous administration of 500 mug/kg to rats revealed that the data best fit a three-compartment open model.
本文描述了一种用于检测血浆中可乐定的高灵敏度气液色谱法及电子捕获检测法。碱性样品（100 - 200微升）用环己烷 - 丁醇（9:1）萃取，再用0.1N硫酸反萃取，碱化后再用环己烷 - 丁醇反萃取。然后形成可乐定和内标2 -（2,4 - 二氯苯基氨基）- 2 - 咪唑啉的五氟苄基衍生物，通过溶剂萃取除去过量试剂。可乐定和内标的最低检测量（MDQ）分别为2.5×10⁻¹⁶摩尔/秒（3.3皮克）和2.8×10⁻¹⁶摩尔/秒（5.9皮克）。血浆样品中能以小于20%标准差的精密度测定的最小可乐定含量为200皮克。给大鼠静脉注射500微克/千克后血浆中可乐定的时间进程表明，数据最符合三室开放模型。
