Chu L C, Bayne W F, Tao F T, Schmitt L G, Shaw J E
J Pharm Sci. 1979 Jan;68(1):72-4. doi: 10.1002/jps.2600680122.
A sensitive and specific GLC method using electron-capture detection was developed for clonidine in plasma and urine. Di-perfluoroacyl derivatives of both clonidine and the 4-methyl analog of clonidine (used as an internal standard) were formed, and an extraction process was developed for the removal of excess derivatization reagent and endogenous biological compounds; the assay permitted quantification of 25 pg of clonidine/ml in a 4-ml plasma sample. The assay was used to elucidate the time course of plasma concentrations in a normotensive subject following oral administration of 50, 100, and 200 microgram of clonidine hydrochloride and also to determine unchanged drug excreted in the urine.
开发了一种灵敏且特异的气相色谱法，采用电子捕获检测测定血浆和尿液中的可乐定。形成了可乐定及其4-甲基类似物（用作内标）的二全氟酰基衍生物，并开发了一种萃取方法以去除过量的衍生化试剂和内源性生物化合物；该测定法能够对4毫升血浆样品中25皮克/毫升的可乐定进行定量。该测定法用于阐明正常血压受试者口服50、100和200微克盐酸可乐定后血浆浓度的时间进程，还用于测定尿液中排泄的未变化药物。
