Padawer J
J Cell Biol. 1969 Mar;40(3):747-60. doi: 10.1083/jcb.40.3.747.
Mast cells from the peritoneal cavity of the rat were obtained at various times following in situ injection of a colloidal thorium dioxide preparation (Thorotrast). They were prepared for electron microscopy by aldehyde fixation, osmium tetroxide postfixation, and embedding in Epon. Thorotrast was rapidly taken up by mast cells through enhanced or newly elicited surface specializations. It was confined at first to large vesicles which moved to the Golgi area. Subsequently, in a matter of a few hours only, it became associated with progressively more mature granules, including "fully" mature ones. In addition to demonstrating a further phagocytic or pinocytotic activity of mast cells, the findings suggest that mast cell granules share a common membranous investment, and that substances from the tissue environment may theoretically percolate over and interact with the granules. Mast cell function could thus be served primarily by absorptive rather than secretory processes.
在原位注射二氧化钍胶体制剂(钍造影剂)后的不同时间,从大鼠腹腔获取肥大细胞。通过醛固定、四氧化锇后固定及包埋于环氧树脂中制备用于电子显微镜观察的样本。钍造影剂通过增强或新引发的表面特化结构被肥大细胞迅速摄取。起初它局限于大囊泡中,这些大囊泡会移动到高尔基体区域。随后,仅在几个小时内,它就与逐渐成熟的颗粒相关联,包括“完全”成熟的颗粒。这些发现除了证明肥大细胞具有进一步的吞噬或胞饮活性外,还表明肥大细胞颗粒共享一个共同的膜性包被,并且来自组织环境的物质理论上可能渗透并与颗粒相互作用。肥大细胞的功能因此可能主要由吸收过程而非分泌过程来完成。