Nielsen E H, Bytzer P, Clausen J, Chakravarty N
Cell Tissue Res. 1981;216(3):635-45. doi: 10.1007/BF00238658.
Regeneration of rat mast cells was studied by TEM from 10 s to 48 h after secretion of histamine induced by compound 48/80. During the first 2 h, small intracellular cavities, formed during compound exocytosis and containing non-membrane-bound remnants of the granules, tended to coalesce, and after 2 h of incubation regeneration started. After 6 h, all the cavities had fused into one large central cavity which contained the remnants of the granules and remained open to the exterior during the entire period. The plasma membrane microfolds which disappeared just after secretion were reformed during regeneration. They were apparently involved in endocytotic-like activity and coated vesicles also appeared beneath the plasmalemma (membrane recycling?). The fate of the granule remnants in the cavity is unknown, as regeneration was not completed after 48 h which is the longest survival time obtained so far in ultrastructural studies of mast cell regeneration in vitro.
通过透射电子显微镜（TEM）研究了化合物48/80诱导组胺分泌后10秒至48小时大鼠肥大细胞的再生情况。在最初的2小时内，复合胞吐过程中形成的、含有颗粒非膜结合残余物的小细胞内腔趋于融合，孵育2小时后再生开始。6小时后，所有的腔融合成一个大的中央腔，其中包含颗粒残余物，并且在整个期间都向外部开放。分泌后立即消失的质膜微褶在再生过程中重新形成。它们显然参与了类似内吞的活动，并且在质膜下方也出现了被膜小泡（膜循环？）。腔内颗粒残余物的命运尚不清楚，因为在48小时后再生尚未完成，这是迄今为止肥大细胞体外再生超微结构研究中获得的最长存活时间。
