电压钳制期间内灌注枪乌贼巨大轴突中的钠内流。
Sodium influxes in internally perfused squid giant axon during voltage clamp.
作者信息
Atwater I, Bezanilla F, Rojas E
出版信息
J Physiol. 1969 May;201(3):657-64. doi: 10.1113/jphysiol.1969.sp008778.
Abstract
- An experimental method for measuring ionic influxes during voltage clamp in the giant axon of Dosidicus is described; the technique combines intracellular perfusion with a method for controlling membrane potential.2. Sodium influx determinations were carried out while applying rectangular pulses of membrane depolarization. The ratio ;measured sodium influx/computed ionic flux during the early current' is 0.92 +/- 0.12.3. Plots of measured sodium influx and computed ionic flux during the early current against membrane potential are very similar. There was evidence that the membrane potential at which the sodium influx vanishes is the potential at which the early current reverses.
摘要
描述了一种在茎柔鱼巨轴突电压钳制期间测量离子内流的实验方法；该技术将细胞内灌注与控制膜电位的方法相结合。
在施加矩形膜去极化脉冲时进行钠内流测定。“测量的钠内流/早期电流期间计算的离子通量”的比值为0.92±0.12。
早期电流期间测量的钠内流和计算的离子通量相对于膜电位的曲线图非常相似。有证据表明钠内流消失时的膜电位是早期电流反转时的电位。
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本文引用的文献
1
The dual effect of membrane potential on sodium conductance in the giant axon of Loligo.枪乌贼巨大轴突中膜电位对钠电导的双重作用。
J Physiol. 1952 Apr;116(4):497-506. doi: 10.1113/jphysiol.1952.sp004719.
2
The components of membrane conductance in the giant axon of Loligo.枪乌贼巨大轴突膜电导的组成成分。
J Physiol. 1952 Apr;116(4):473-96. doi: 10.1113/jphysiol.1952.sp004718.
3
Currents carried by sodium and potassium ions through the membrane of the giant axon of Loligo.钠和钾离子通过枪乌贼巨大轴突膜所携带的电流。
J Physiol. 1952 Apr;116(4):449-72. doi: 10.1113/jphysiol.1952.sp004717.
4
The ionic movements during nervous activity.神经活动期间的离子运动。
J Physiol. 1951 Jun;114(1-2):119-50. doi: 10.1113/jphysiol.1951.sp004608.
5
Sodium and potassium ion effluxes from squid axons under voltage clamp conditions.电压钳制条件下乌贼轴突中钠和钾离子的外流。
Biophys J. 1962 May;2(3):257-74. doi: 10.1016/s0006-3495(62)86854-4.
6
ION FLUXES AND TRANSFERENCE NUMBER IN SQUID AXONS.乌贼轴突中的离子通量与迁移数
J Neurophysiol. 1965 May;28:526-44. doi: 10.1152/jn.1965.28.3.526.
7
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Movement of radioactive potassium and membrane current in a giant axon.放射性钾在巨轴突中的移动及膜电流
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