电压钳制期间内灌注枪乌贼巨大轴突中的钠内流。
Sodium influxes in internally perfused squid giant axon during voltage clamp.
作者信息
Atwater I, Bezanilla F, Rojas E
出版信息
J Physiol. 1969 May;201(3):657-64. doi: 10.1113/jphysiol.1969.sp008778.
Abstract
- An experimental method for measuring ionic influxes during voltage clamp in the giant axon of Dosidicus is described; the technique combines intracellular perfusion with a method for controlling membrane potential.2. Sodium influx determinations were carried out while applying rectangular pulses of membrane depolarization. The ratio ;measured sodium influx/computed ionic flux during the early current' is 0.92 +/- 0.12.3. Plots of measured sodium influx and computed ionic flux during the early current against membrane potential are very similar. There was evidence that the membrane potential at which the sodium influx vanishes is the potential at which the early current reverses.
摘要
描述了一种在茎柔鱼巨轴突电压钳制期间测量离子内流的实验方法；该技术将细胞内灌注与控制膜电位的方法相结合。
在施加矩形膜去极化脉冲时进行钠内流测定。“测量的钠内流/早期电流期间计算的离子通量”的比值为0.92±0.12。
早期电流期间测量的钠内流和计算的离子通量相对于膜电位的曲线图非常相似。有证据表明钠内流消失时的膜电位是早期电流反转时的电位。
相似文献
1
Sodium influxes in internally perfused squid giant axon during voltage clamp.
J Physiol. 1969 May;201(3):657-64. doi: 10.1113/jphysiol.1969.sp008778.
2
Time course of the sodium influx in squid giant axon during a single voltage clamp pulse.
J Physiol. 1970 Mar;207(1):151-64. doi: 10.1113/jphysiol.1970.sp009054.
3
Simultaneous measurements of magnesium, calcium and sodium influxes in perfused squid giant axons under membrane potential control.
J Physiol. 1975 Oct;252(1):1-27. doi: 10.1113/jphysiol.1975.sp011131.
4
Effect of stimulation and hyperpolarization on non-electrolyte and sodium permeability in perfused axons of squid.
J Physiol. 1970 Nov;211(1):193-202. doi: 10.1113/jphysiol.1970.sp009274.
5
Calcium influxes in perfused squid giant axons during voltage clamp.
J Physiol. 1970 Sep;210(2):135P.
6
Comparison of tetrodotoxin and procaine in internally perfused squid giant axons.
J Gen Physiol. 1967 May;50(5):1413-28. doi: 10.1085/jgp.50.5.1413.
7
Sodium movements in perfused squid giant axons. Passive fluxes.
J Gen Physiol. 1968 Aug;52(2):240-57. doi: 10.1085/jgp.52.2.240.
8
The action of phospholipases on the inner and outer surface of the squid giant axon membrane.
J Physiol. 1972 Jan;220(1):73-86. doi: 10.1113/jphysiol.1972.sp009695.
9
Cation interdiffusion in squid giant axons.
J Gen Physiol. 1967 Mar;50(4):989-1007. doi: 10.1085/jgp.50.4.989.
10
Effects of batrachotoxin on membrane potential and conductance of squid giant axons.
J Gen Physiol. 1971 Jul;58(1):54-70. doi: 10.1085/jgp.58.1.54.
引用本文的文献
1
Influences: The Cell Physiology Laboratory in Montemar, Chile.
J Gen Physiol. 2018 Nov 5;150(11):1464-1468. doi: 10.1085/jgp.201812157. Epub 2018 Oct 19.
2
Time course of the sodium influx in squid giant axon during a single voltage clamp pulse.
J Physiol. 1970 Mar;207(1):151-64. doi: 10.1113/jphysiol.1970.sp009054.
3
Sodium and potassium conductance changes during a membrane action potential.
J Physiol. 1970 Dec;211(3):729-51. doi: 10.1113/jphysiol.1970.sp009301.
4
Time course of the sodium permeability change during a single membrane action potential.
J Physiol. 1970 Dec;211(3):753-65. doi: 10.1113/jphysiol.1970.sp009302.
5
Sodium and potassium currents in squid axons perfused with fluoride solutions.
J Physiol. 1970 Dec;211(3):623-52. doi: 10.1113/jphysiol.1970.sp009297.
6
Effect of stimulation and hyperpolarization on non-electrolyte and sodium permeability in perfused axons of squid.
J Physiol. 1970 Nov;211(1):193-202. doi: 10.1113/jphysiol.1970.sp009274.
7
Direct and rapid description of the individual ionic currents of squid axon membrane by ramp potential control.
Biophys J. 1970 Sep;10(9):799-817. doi: 10.1016/S0006-3495(70)86336-6.
8
Depolarization and calcium entry in squid giant axons.
J Physiol. 1971 Nov;218(3):709-55. doi: 10.1113/jphysiol.1971.sp009641.
9
The permeability of the sodium channel to metal cations in myelinated nerve.
J Gen Physiol. 1972 Jun;59(6):637-58. doi: 10.1085/jgp.59.6.637.
10
The temperature dependence of the movement of sodium ions associated with nerve impulses.
J Physiol. 1974 Jan;236(1):95-111. doi: 10.1113/jphysiol.1974.sp010424.
本文引用的文献
1
The dual effect of membrane potential on sodium conductance in the giant axon of Loligo.
J Physiol. 1952 Apr;116(4):497-506. doi: 10.1113/jphysiol.1952.sp004719.
2
The components of membrane conductance in the giant axon of Loligo.
J Physiol. 1952 Apr;116(4):473-96. doi: 10.1113/jphysiol.1952.sp004718.
3
Currents carried by sodium and potassium ions through the membrane of the giant axon of Loligo.
J Physiol. 1952 Apr;116(4):449-72. doi: 10.1113/jphysiol.1952.sp004717.
4
The ionic movements during nervous activity.
J Physiol. 1951 Jun;114(1-2):119-50. doi: 10.1113/jphysiol.1951.sp004608.
5
Sodium and potassium ion effluxes from squid axons under voltage clamp conditions.
Biophys J. 1962 May;2(3):257-74. doi: 10.1016/s0006-3495(62)86854-4.
6
ION FLUXES AND TRANSFERENCE NUMBER IN SQUID AXONS.
J Neurophysiol. 1965 May;28:526-44. doi: 10.1152/jn.1965.28.3.526.
7
Kinetics of ion movement in the squid giant axon.
J Gen Physiol. 1955 Nov 20;39(2):279-300. doi: 10.1085/jgp.39.2.279.
8
Movement of radioactive potassium and membrane current in a giant axon.
J Physiol. 1953 Aug;121(2):403-14. doi: 10.1113/jphysiol.1953.sp004954.
9
Voltage clamp experiments on internally perfused giant axons.
J Physiol. 1965 Oct;180(4):788-820. doi: 10.1113/jphysiol.1965.sp007732.
10
Sodium movements in perfused squid giant axons. Passive fluxes.
J Gen Physiol. 1968 Aug;52(2):240-57. doi: 10.1085/jgp.52.2.240.
Zotero 插件