通过斜坡电位控制直接快速描述鱿鱼轴突膜的单个离子电流。
Direct and rapid description of the individual ionic currents of squid axon membrane by ramp potential control.
作者信息
Fishman H M
出版信息
Biophys J. 1970 Sep;10(9):799-817. doi: 10.1016/S0006-3495(70)86336-6.
Abstract
Computations based upon the Hodgkin-Huxley equations and experimental data from squid axons show that ramp functions can be used as commands to a voltage clamp system to selectively observe either the fast (sodium) or slow (potassium) process in axon membranes without chemical separation techniques or computer assistance. Each process is characterized directly (on line) and rapidly (real time) by generating a current-potential curve on an oscilloscope for fast or slow rates of change of membrane potential (ramps). The speed and directness of this method of characterizing each of the essential axonal events permit quantitative measurement of the kinetics of rapid effects on these processes due to various pharmacological agents such as tetrodotoxin and tetraethylammonium ion or other experimental changes in the membrane environment.
摘要
基于霍奇金-赫胥黎方程的计算以及来自鱿鱼轴突的实验数据表明，斜坡函数可用作电压钳系统的指令，以在无需化学分离技术或计算机辅助的情况下，选择性地观察轴突膜中的快速（钠）或慢速（钾）过程。通过在示波器上生成膜电位快速或慢速变化（斜坡）的电流-电位曲线，可直接（在线）且快速（实时）地对每个过程进行表征。这种表征每个基本轴突事件的方法的速度和直接性，使得能够定量测量各种药理试剂（如河豚毒素和四乙铵离子）或膜环境中的其他实验变化对这些过程的快速影响的动力学。
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ELECTRIC IMPEDANCE OF THE SQUID GIANT AXON DURING ACTIVITY.超导巨轴突在活动期间的电阻抗。
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