单个膜动作电位期间钠通透性变化的时间进程。
Time course of the sodium permeability change during a single membrane action potential.
作者信息
Atwater I, Bezanilla F, Rojas E
出版信息
J Physiol. 1970 Dec;211(3):753-65. doi: 10.1113/jphysiol.1970.sp009302.
Abstract
- A method for measuring the time course of ionic fluxes during a non-propagated membrane action potential is described; the technique combines intracellular perfusion of the squid giant axon with radioactive tracer methods and with a method for controlling the membrane potential during small time intervals.2. The method is used to determine the temporal course of the sodium extra influx during an action potential.3. The results agree with the time course of the permeability change to sodium ions calculated with the Hodgkin & Huxley equations.4. An average extra sodium influx of 7.13 p-mole/cm(2) per action potential was determined at 12 degrees C and of 5.23 p-mole/cm(2) per action potential at 15 degrees C.
摘要
本文描述了一种测量非传播性膜动作电位期间离子通量时间进程的方法；该技术将乌贼巨大轴突的细胞内灌注与放射性示踪方法以及在短时间间隔内控制膜电位的方法相结合。
该方法用于确定动作电位期间钠额外内流的时间进程。
结果与用霍奇金和赫胥黎方程计算的钠离子通透性变化的时间进程一致。
在12℃时，每个动作电位的平均额外钠内流为7.13皮摩尔/平方厘米，在15℃时为5.23皮摩尔/平方厘米。
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本文引用的文献
1
The sodium and potassium content of cephalopod nerve fibers.头足类神经纤维的钠和钾含量。
J Physiol. 1951 Jun;114(1-2):151-82. doi: 10.1113/jphysiol.1951.sp004609.
2
ANOMALOUS RECTIFICATION IN THE SQUID GIANT AXON INJECTED WITH TETRAETHYLAMMONIUM CHLORIDE.注射氯化四乙铵的乌贼巨轴突中的反常整流
J Gen Physiol. 1965 May;48(5):859-72. doi: 10.1085/jgp.48.5.859.
3
A quantitative description of membrane current and its application to conduction and excitation in nerve.膜电流的定量描述及其在神经传导和兴奋中的应用。
J Physiol. 1952 Aug;117(4):500-44. doi: 10.1113/jphysiol.1952.sp004764.
4
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J Gen Physiol. 1966 Nov;50(2):491-503. doi: 10.1085/jgp.50.2.491.
5
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J Gen Physiol. 1967 May;50(5):1287-302. doi: 10.1085/jgp.50.5.1287.
6
Cation movements in perfused giant axons.灌注大神经轴突中的阳离子运动。
J Physiol. 1966 Jan;182(1):209-16. doi: 10.1113/jphysiol.1966.sp007819.
7
Analysis of the effects of calcium or magnesium on voltage-clamp currents in perfused squid axons bathed in solutions of high potassium.分析钙或镁对浸泡在高钾溶液中的灌注枪乌贼轴突电压钳电流的影响。
J Gen Physiol. 1969 Oct;54(4):532-52. doi: 10.1085/jgp.54.4.532.
8
Sodium influxes in internally perfused squid giant axon during voltage clamp.电压钳制期间内灌注枪乌贼巨大轴突中的钠内流。
J Physiol. 1969 May;201(3):657-64. doi: 10.1113/jphysiol.1969.sp008778.
9
Sodium movements in perfused squid giant axons. Passive fluxes.灌注乌贼巨大轴突中的钠运动。被动通量。
J Gen Physiol. 1968 Aug;52(2):240-57. doi: 10.1085/jgp.52.2.240.
10
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J Physiol. 1970 Mar;207(1):151-64. doi: 10.1113/jphysiol.1970.sp009054.
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