通过磷酸钙柱层析法纯化和浓缩甲型和乙型流感病毒。
Purification and concentration of influenza types A and B by chromatography on calcium phosphate.
作者信息
Lapidus M
出版信息
Appl Microbiol. 1969 Apr;17(4):504-6. doi: 10.1128/am.17.4.504-506.1969.
Abstract
A rapid chromatographic method for the isolation of types A and B influenza virus from allantoic fluid was described. The adsorbent was prepared from calcium dihydrogen orthophosphate monohydrate [Ca(H(2)PO(4))(2).H(2)O] by alkali treatment. The addition of sodium trimetaphosphate to the influenza-infected allantoic fluid afforded a 67 to 100% viral recovery and a 26 to 43-fold increase in purity.
摘要
描述了一种从尿囊液中分离甲型和乙型流感病毒的快速色谱方法。吸附剂由一水磷酸二氢钙[Ca(H₂PO₄)₂·H₂O]经碱处理制备而成。向感染流感病毒的尿囊液中添加三聚磷酸钠，病毒回收率为67%至100%，纯度提高了26至43倍。
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