Brunner M, Pootjes C F
J Virol. 1969 Feb;3(2):181-6. doi: 10.1128/JVI.3.2.181-186.1969.
Bacteriophage release in a lysogenic strain of Agrobacterium tumefaciens V-1 is temperature-sensitive. At 25 C and 30 C, phage was released in a ratio of 1 plaque-forming unit per 100 bacteria; at 35 C, although bacterial growth was not inhibited, phage release was suppressed. Phage synthesis was induced by heat shock, 42 C for 30 min, ultraviolet irradiation, and mitomycin C. Induction by ultraviolet light was unusual-an immediate rise in phage titer followed irradiation. A large increase occurred after a 90-min latent period. The lysogenic strain was cured of the phage by incubation at 37 C, and the cured strain produced plant tumors.
根癌土壤杆菌V-1溶源菌株中的噬菌体释放对温度敏感。在25℃和30℃时，噬菌体以每100个细菌1个噬菌斑形成单位的比例释放；在35℃时，尽管细菌生长未受抑制，但噬菌体释放受到抑制。噬菌体合成可通过热休克（42℃处理30分钟）、紫外线照射和丝裂霉素C诱导。紫外线诱导的情况不同寻常——照射后噬菌体效价立即上升。90分钟的潜伏期后出现大幅增加。通过在37℃培养可使溶源菌株的噬菌体治愈，治愈后的菌株会产生植物肿瘤。
