Vervliet G, Holsters M, Teuchy H, Van Montagu M, Schell J
J Gen Virol. 1975 Jan;26(1):33-48. doi: 10.1099/0022-1317-26-1-33.
Four Agrobacterium tumefaciens temperate phages (PB2A, PB6(omega), PV-1(LV-1) and PS8), were shown to have the same genome size. Moreover hybridization experiments by the heteroduplex method and electron microscopy showed a 100% homology between these four phage genomes. Indications for lysogeny were found by direct means for the Agrobacterum timefaciens strain 396, Agrobacterium radiobacter strain 8149 and Agrobacterium species 0362 and by the electron microscope negative staining technique for the A.tumefaciens strains b6-806,b6-6,b6s3,b2as,cv-1,4452,11156,11158,396, and 925; for A.radiobacter strains tr-1 and 8149, the latter being bi-lysogenic, and for the A. species 0362. These isolated phage particles, most of which appear to be defective, could be grouped into different classes. No particles could be detected in the lysates of A. tumefaciens RV3, A. radiobacter strains 4718 and S1005, and A. species 0363. Further characterization by genome size was carried out for the defective temperate phages PB6-806, P4452,P8149 and P0362. No evidence for homology between PB6-806 and PB6 omega could be found. The defective phages PB6-806 and P4452 showed the same morphology but a different genome size, whereas the two phages P0362 and P8149 had a very different morphology and genome size.
四种根癌土壤杆菌温和噬菌体（PB2A、PB6(ω)、PV-1(LV-1)和PS8）的基因组大小相同。此外，通过异源双链法和电子显微镜进行的杂交实验表明，这四种噬菌体基因组之间具有100%的同源性。通过直接方法在根癌土壤杆菌菌株396、放射土壤杆菌菌株8149和土壤杆菌属0362中发现了溶原性迹象，通过电子显微镜负染色技术在根癌土壤杆菌菌株b6-806、b6-6、b6s3、b2as、cv-1、4452、11156、11158、396和925中也发现了溶原性迹象；在放射土壤杆菌菌株tr-1和8149（后者为双溶原菌）以及土壤杆菌属0362中也发现了溶原性迹象。这些分离出的噬菌体颗粒大多似乎有缺陷，可分为不同类别。在根癌土壤杆菌RV3、放射土壤杆菌菌株4718和S1005以及土壤杆菌属0363的裂解物中未检测到颗粒。对缺陷型温和噬菌体PB6-806、P4452、P8149和P0362进行了基因组大小的进一步表征。未发现PB6-806和PB6 ω之间存在同源性的证据。缺陷噬菌体PB6-806和P4452形态相同，但基因组大小不同，而噬菌体P0362和P8149的形态和基因组大小则有很大差异。
