大脑中的一个磷酸化位点以及某些有机磷化合物的迟发性神经毒性作用。
A phosphorylation site in brain and the delayed neurotoxic effect of some organophosphorus compounds.
作者信息
Johnson M K
出版信息
Biochem J. 1969 Feb;111(4):487-95. doi: 10.1042/bj1110487.
Abstract
- It is proposed that part of a neurotoxic dose of di-isopropyl phosphorofluoridate will be covalently bound in vivo to a specific component in the brain and spinal cord as the initial biochemical event in the genesis of the lesion. 2. A test system in vitro was devised that removes many di-isopropyl phosphorofluoridate-binding sites and indicates that the specific component may be a protein present in brain at a concentration comparable with that of the cholinesterases. 3. The site was found to be present and capable of binding di-isopropyl phosphorofluoridate in vitro in brain samples taken from either normal hens or those dosed with organophosphorus esterase inhibitors that are not neurotoxic. 4. Very little of the specific binding activity was found in brain samples from hens pre-dosed with a variety of neurotoxic organophosphorus compounds. 5. A solubilized preparation of the active brain component was obtained, suitable for further purification and study.
摘要
- 有人提出，神经毒性剂量的二异丙基氟磷酸酯的一部分会在体内与脑和脊髓中的特定成分共价结合，这是损伤发生过程中的初始生化事件。2. 设计了一种体外测试系统，该系统去除了许多二异丙基氟磷酸酯结合位点，并表明该特定成分可能是脑中存在的一种蛋白质，其浓度与胆碱酯酶相当。3. 发现该位点在取自正常母鸡或用无神经毒性的有机磷酸酯酶抑制剂给药的母鸡的脑样本中存在且能够在体外结合二异丙基氟磷酸酯。4. 在预先用多种神经毒性有机磷化合物给药的母鸡的脑样本中发现的特异性结合活性非常少。5. 获得了活性脑成分的可溶制剂，适合进一步纯化和研究。
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