Randall C C, Gafford L G, Gentry G A, Lawson L A
Science. 1965 Sep 3;149(3688):1098-9. doi: 10.1126/science.149.3688.1098.
In HeLa-cell cultures chronically infected with Mycoplasma, (PPLO) host-cell DNA is unstable as detected by incorporation of H(3)-or C1 i-thymidine into DNA and subsequent release into the medium as acidsoluble radioactivity. This characteristic can be transmitted to PPLO-free cultures of strain L cells by inoculation with preparations of PPLO from the HeLa cells, although chronically infected cultures of L cells continue to multiply. In addition, virus preparations also may carry PPLO contamination through numerous passages.
在长期感染支原体（类胸膜肺炎微生物）的海拉细胞培养物中，通过将H(3)-或C1-胸腺嘧啶核苷掺入DNA并随后作为酸溶性放射性物质释放到培养基中检测到，宿主细胞DNA不稳定。这种特性可以通过接种来自海拉细胞的类胸膜肺炎微生物制剂传递给L细胞系的无类胸膜肺炎微生物培养物，尽管L细胞的长期感染培养物继续增殖。此外，病毒制剂在多次传代过程中也可能携带类胸膜肺炎微生物污染。
