Njio K D, Peters J M
Acta Endocrinol (Copenh). 1977 Nov;86(3):539-51. doi: 10.1530/acta.0.0860539.
Mammary gland explants obtained from 13 days pregnant rats were cultured for 3, 6 and 9 days in the presence of serum of either virgin rats or 13 days pregnant rats. Insulin was added alone or in combination with cortisol. The way these different forms of treatment affected the ultrastructure was studied. With virgin rat serum plus insulin alone the mammary gland regressed to a lower state of development comparable to that of the virgin rat in vivo. When virgin rat serum was replaced by 13 days pregnant rat serum--which contains rat chorionic mammotrophin--the initial development was maintained, but synthesis of secretory products ceased. When cortisol was added to the virgin rat serum plus insulin, cortisol maintained the differentiated state originally present. Even some secretory activity was induced, but at a minimal level. With cortisol added to the pregnant rat serum plus insulin, the explants were stimulated to synthetize the extrude secretion products.
从怀孕13天的大鼠获取乳腺外植体，在未孕大鼠或怀孕13天大鼠的血清存在下培养3天、6天和9天。单独添加胰岛素或与皮质醇联合添加。研究了这些不同形式的处理对超微结构的影响。仅使用未孕大鼠血清加胰岛素时，乳腺退化到与体内未孕大鼠相当的较低发育状态。当用含有大鼠绒毛膜促乳腺素的怀孕13天大鼠血清替代未孕大鼠血清时，最初的发育得以维持，但分泌产物的合成停止。当向未孕大鼠血清加胰岛素中添加皮质醇时，皮质醇维持了最初存在的分化状态。甚至诱导了一些分泌活性，但水平极低。当向怀孕大鼠血清加胰岛素中添加皮质醇时，外植体被刺激合成并挤出分泌产物。
