Ford W L
Br J Exp Pathol. 1969 Jun;50(3):257-69.
The great majority of the cells released by the isolated, perfused rat spleen were lymphocytes of which about 95 per cent were small lymphocytes. The rate of release of small lymphocytes from the spleen was independent of the prevailing concentration in the perfusate. The cells released by the spleen were immunologically competent in respect of a graft-versus-host reaction and a primary antibody response. They could also transfer secondary responsiveness to a viral antigen. Several spleen donors were given a continuous intravenous infusion of tritiated thymidine prior to spleen perfusion and the proportion of labelled small lymphocytes among the population released by the spleen was compared to the proportion in populations of small lymphocytes from other sources. The small lymphocytes released by the spleen recirculated from the blood to thoracic duct lymph after injection into a syngeneic recipient. Conversely the perfused spleen released thoracic duct small lymphocytes which had been given to the spleen donor 24 hr previously. It is therefore probable that a single population of recirculating small lymphocytes exists which migrates from the blood into both lymph-nodes and spleen. The release of small lymphocytes from the spleen was mostly at the expense of the lymphocyte content of the periarteriolar lymphoid sheaths.
分离灌注的大鼠脾脏释放的细胞绝大多数是淋巴细胞，其中约95%是小淋巴细胞。脾脏释放小淋巴细胞的速率与灌注液中当前的浓度无关。脾脏释放的细胞在移植物抗宿主反应和初次抗体反应方面具有免疫活性。它们还能传递对病毒抗原的二次反应性。在脾脏灌注前，给几只脾脏供体连续静脉输注氚标记的胸腺嘧啶核苷，并将脾脏释放的细胞群体中标记的小淋巴细胞比例与其他来源的小淋巴细胞群体中的比例进行比较。脾脏释放的小淋巴细胞注射到同基因受体中后，会从血液再循环到胸导管淋巴中。相反，灌注的脾脏会释放24小时前给予脾脏供体的胸导管小淋巴细胞。因此，很可能存在单一的再循环小淋巴细胞群体，它们从血液迁移到淋巴结和脾脏中。脾脏释放小淋巴细胞主要是以动脉周围淋巴鞘中的淋巴细胞含量为代价的。
