Kalpaktsoglou P K, Yunis E J, Good R A
Clin Exp Immunol. 1969 Jul;5(1):91-103.
The effect of thymectomy and splenectomy in C3H/Bi mice on the responses of circulating leucocytes and on morphological changes of the haematopoietic tissues after injection of pertussis vaccine has been studied. After pertussis all mice showed depletion of lymphoid cells in all the lymphoid organs as well as in bone-marrow and an increased number of leucocytes, lymphocytes, neutrophils and monocytes in the circulation. Neonatal thymectomy decreased lymphocytosis produced by pertussis. Thymectomy, at all ages studied, fostered an increase in the number of monocytes and polymorphonuclears in circulation. Splenectomy at birth or early in life provoked an increase in levels of circulating polymorphonuclears and lymphocytes in pertussis treated animals. In neonatally thymectomized mice the depletion of lymphoid cells from lymphoid tissues after pertussis could be shown to include the thymic-independent areas. The depletion of small lymphocytes from thymus following pertussis persisted longer than depletion of small lymphocytes from spleen, marrow or lymph nodes. The longer persistence of lymphoid depletion in the thymus than in peripheral lymphoid tissues is, we believe, to be related to the central lymphoid function of thymus as a site of differentiation of lymphoid cells and to the aloofness of thymus from recirculation of fully differentiated peripheral lymphocytes.
研究了切除C3H/Bi小鼠的胸腺和脾脏对注射百日咳疫苗后循环白细胞反应及造血组织形态变化的影响。注射百日咳疫苗后，所有小鼠的所有淋巴器官以及骨髓中的淋巴细胞均减少，循环中的白细胞、淋巴细胞、中性粒细胞和单核细胞数量增加。新生期胸腺切除可减少百日咳引起的淋巴细胞增多。在所研究的所有年龄段，胸腺切除均促使循环中单核细胞和多形核细胞数量增加。出生时或生命早期进行脾脏切除，可使接受百日咳治疗的动物循环中的多形核细胞和淋巴细胞水平升高。在新生期胸腺切除的小鼠中，百日咳后淋巴组织中淋巴细胞的减少可显示包括非胸腺依赖区。百日咳后胸腺中小淋巴细胞的减少持续时间比脾脏、骨髓或淋巴结中小淋巴细胞的减少持续时间更长。我们认为，胸腺中淋巴细胞减少的持续时间比外周淋巴组织中更长，这与胸腺作为淋巴细胞分化部位的中枢淋巴功能以及胸腺与完全分化的外周淋巴细胞再循环的隔离有关。
