Kadota T, Kadota K, Gray E G
J Cell Biol. 1976 Jun;69(3):608-21. doi: 10.1083/jcb.69.3.608.
Coated vesicles (CVs), plain synaptic vesicles (PSVs), and nonvesicular flocculent material were isolated from synaptosomes and examined with goniometry and high-resolution electron microscopy after either negative staining or various biochemical procedures. The flocculent material (i.e. the presynaptic matrix material except CV shells) is largely composed of particulate or elongated (chainlike) structures; some of this material (here referred to as particle/chain material) is attached to PSVs. The results obtained were: (a) the proteinaceous properties of the CV coat (also referred to as CV shell) and the particle/chain material were demonstrated with chymotrypsin; (b) the CV shell, studied with various negative-staining techniques, differs from the particle/chain material since it has no 3-4-nm globular subunits and reacts differently to alkaline pH; (c) the particle/chain material consists of aggregates of 3-4-nm globular subunits, four of which yield 8-10-nm fine particles; and these particles can be further aggregated into chains 8-10 nm wide and up to 30-60 nm long showing a "hollow" core; (d) vinblastine sulfate induced ringlike or helical crystalloid precipitates closely resembling the vinblastine-induced microtubule crystals reported in the literature, but vinblastine had no effect on either the CV shell material or the particle/chain material.
从突触体中分离出包被小泡（CVs）、普通突触小泡（PSVs）和非小泡状絮状物质，并在负染色或各种生化处理后，用测角术和高分辨率电子显微镜进行检查。絮状物质（即除CV外壳外的突触前基质物质）主要由颗粒状或细长的（链状）结构组成；这种物质中的一些（这里称为颗粒/链物质）附着在PSVs上。得到的结果如下：（a）用胰凝乳蛋白酶证实了CV包被（也称为CV外壳）和颗粒/链物质的蛋白质性质；（b）用各种负染色技术研究的CV外壳与颗粒/链物质不同，因为它没有3-4纳米的球状亚基，并且对碱性pH的反应不同；（c）颗粒/链物质由3-4纳米球状亚基的聚集体组成，其中四个产生8-10纳米的细颗粒；这些颗粒可以进一步聚集成宽8-10纳米、长达30-60纳米的链，显示出“中空”核心；（d）硫酸长春碱诱导出环状或螺旋状晶体沉淀物，与文献中报道的长春碱诱导的微管晶体非常相似，但长春碱对CV外壳物质或颗粒/链物质均无影响。
