Zheng Qinghua, Guo Lina, Huang Jianyan, Hao Xinyuan, Li Xiaoman, Li Nana, Wang Yueqi, Zhang Kexin, Wang Xinchao, Wang Lu, Zeng Jianming
Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, National Center for Tea Plant Improvement, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China.
Front Plant Sci. 2023 Oct 2;14:1268537. doi: 10.3389/fpls.2023.1268537. eCollection 2023.
Tea plants () show discrepancies in selenium accumulation and transportation, the molecular mechanisms of which are not well understood. Hence, we aimed to conduct a systematic investigation of selenium accumulation and transportation mechanisms in different tea cultivars via transcriptome analysis. The NaSeO and NaSeO treatments improved selenium contents in the roots and leaves of three tea cultivars. The high selenium-enrichment ability (HSe) tea cultivars accumulated higher selenium contents in the leaves than did the low selenium-enrichment ability (LSe) tea cultivars. Transcriptome analysis revealed that differentially expressed genes (DEGs) under the NaSeO and NaSeO treatments were enriched in flavonoid biosynthesis in leaves. DEGs under the NaSeO treatment were enriched in glutathione metabolism in the HSe tea cultivar roots compared to those of the LSe tea cultivar. More transporters and transcription factors involved in improving selenium accumulation and transportation were identified in the HSe tea cultivars under the NaSeO treatment than in the NaSeO treatment. In the HSe tea cultivar roots, the expression of sulfate transporter 1;2 () and increased in response to NaSeO exposure. In contrast, ATP-binding cassette transporter genes (s), glutathione -transferase genes (s), phosphate transporter 1;3 (), nitrate transporter 1 (), and 34 transcription factors were upregulated in the presence of NaSeO. In the HSe tea cultivar leaves, ATP-binding cassette subfamily B member 11 () and 14 transcription factors were upregulated under the NaSeO treatment. Among them, was explored as a potential transcription factor that regulated the accumulation of NaSeO in the roots of HSe tea cultivars. This study preliminary clarified the mechanism of selenium accumulation and transportation in tea cultivars, and the findings have important theoretical significance for the breeding and cultivation of selenium-enriched tea cultivars.
茶树（）在硒积累和转运方面存在差异，其分子机制尚不清楚。因此，我们旨在通过转录组分析对不同茶树品种中硒积累和转运机制进行系统研究。亚硒酸钠（NaSeO）和硒酸钠（NaSeO）处理提高了三个茶树品种根和叶中的硒含量。高硒富集能力（HSe）的茶树品种叶片中积累的硒含量高于低硒富集能力（LSe）的茶树品种。转录组分析表明，亚硒酸钠和硒酸钠处理下的差异表达基因（DEGs）在叶片黄酮类生物合成中富集。与LSe茶树品种相比，亚硒酸钠处理下HSe茶树品种根中的DEGs在谷胱甘肽代谢中富集。与亚硒酸钠处理相比，在亚硒酸钠处理下的HSe茶树品种中鉴定出更多参与改善硒积累和转运的转运蛋白和转录因子。在HSe茶树品种根中，硫酸根转运蛋白1;2（）和的表达响应亚硒酸钠暴露而增加。相反，在存在亚硒酸钠的情况下，ATP结合盒转运蛋白基因（s）、谷胱甘肽 - 转移酶基因（s）、磷酸盐转运蛋白1;3（）、硝酸盐转运蛋白1（）和34个转录因子上调。在HSe茶树品种叶片中，ATP结合盒亚家族B成员11（）和14个转录因子在亚硒酸钠处理下上调。其中，被探索为调节HSe茶树品种根中硒酸钠积累的潜在转录因子。本研究初步阐明了茶树品种中硒积累和转运的机制，研究结果对富硒茶树品种的选育和栽培具有重要的理论意义。
