Fedorko M E, Levine R F
J Histochem Cytochem. 1976 Apr;24(4):601-5. doi: 10.1177/24.4.58024.
Plasma membranes in isolated guinea pig megakaryocytes and washed platelets are poorly stained with the usual methods used to outline cell membranes. The addition of tannic acid and calcium to the initial fixative is useful to enhance electron density of all surface-derived membrane systems in these cells. The method described here shows that the increased electron denisty of membrane after fixation in the presence of tannic acid occurs both at the cell surface and along the invaginated membrane systems.
分离出的豚鼠巨核细胞和洗涤后的血小板中的质膜，用通常用于勾勒细胞膜的方法染色效果不佳。在初始固定剂中添加单宁酸和钙，有助于增强这些细胞中所有源自表面的膜系统的电子密度。此处描述的方法表明，在单宁酸存在下固定后，膜的电子密度增加既发生在细胞表面，也发生在凹陷的膜系统中。
