Barnes I J, Bondi A, Moat A G
J Bacteriol. 1969 Jul;99(1):169-74. doi: 10.1128/jb.99.1.169-174.1969.
Lysine biosynthesis in Staphylococcus aureus has been studied by use of a series of lysine auxotrophs. The strains were isolated after chemical mutagenesis. The majority of these mutant strains were classified according to the enzymatic step found to be deficient. Specific enzyme assays as well as nutritional tests were used to group the organisms. The enzymes included were dihydrodipicolinate synthetase, dihydrodipicolinate reductase, diaminopimelate epimerase, and diaminopimelate decarboxylase. The accumulation of diaminopimelate in certain mutants and the demonstration of dihydrodipicolinate synthetase and reductase provide the first detailed evidence that S. aureus utilizes the diaminopimelate pathway for lysine biosynthesis. A cell-free system was used to study the regulation of these enzymes with the exception of diaminopimelate epimerase. Lysine repressed all of the enzymes tested. The repression appeared to be coordinate in nature. The data presented provide suggestive evidence that the lysine biosynthetic region in S. aureus constitutes an operon.
通过使用一系列赖氨酸营养缺陷型菌株，对金黄色葡萄球菌中的赖氨酸生物合成进行了研究。这些菌株是在化学诱变后分离得到的。这些突变菌株中的大多数是根据发现有缺陷的酶促步骤进行分类的。使用特定的酶测定以及营养测试对这些生物体进行分组。所涉及的酶包括二氢吡啶二羧酸合成酶、二氢吡啶二羧酸还原酶、二氨基庚二酸差向异构酶和二氨基庚二酸脱羧酶。某些突变体中二氨基庚二酸的积累以及二氢吡啶二羧酸合成酶和还原酶的证明，提供了首个详细证据，表明金黄色葡萄球菌利用二氨基庚二酸途径进行赖氨酸生物合成。除二氨基庚二酸差向异构酶外，使用无细胞系统来研究这些酶的调控。赖氨酸抑制了所有测试的酶。这种抑制在本质上似乎是协同的。所呈现的数据提供了暗示性证据，表明金黄色葡萄球菌中的赖氨酸生物合成区域构成一个操纵子。
