Raine C S, Feldman L A, Sheppard R D, Bornstein M B
J Virol. 1969 Aug;4(2):169-81. doi: 10.1128/JVI.4.2.169-181.1969.
Replication of Edmonston strain of measles virus in cultures of hamster central nervous system tissue was studied by electron microscopy of ultrathin sections. Infected cultures were fixed from 3 hr to 39 days postinoculation (PI). Measles nucleocapsid was first seen within the cytoplasm of giant cells, the latter appearing 5 to 6 days PI. Measles virus particles were most abundant at 10 days PI and appeared to bud off from areas of the cell membrane along which nucleocapsid was aligned. Intranuclear nucleocapsid was more abundant at later stages, and by 39 days PI entire nuclei were seen to be occupied. By this time, the cytoplasmic formations, which had been sequestered by membranes, appeared to lose their regular structure. Budding viral particles at 39 days PI were of a much simplified structure and did not involve the alignment of nucleocapsid about their periphery.
通过超薄切片电子显微镜观察，研究了麻疹病毒埃德蒙斯顿株在仓鼠中枢神经系统组织培养物中的复制情况。在接种后3小时至39天对感染的培养物进行固定。麻疹核衣壳最早在巨细胞的细胞质中可见，巨细胞在接种后5至6天出现。麻疹病毒颗粒在接种后10天最为丰富，似乎从核衣壳排列的细胞膜区域出芽。核内核衣壳在后期更为丰富，到接种后39天，整个细胞核都被占据。此时，被膜隔离的细胞质结构似乎失去了其规则结构。接种后39天出芽的病毒颗粒结构更为简单，且其周围没有核衣壳排列。
