胚胎期鸡软骨中硫酸软骨素的硫酸化作用
The sulphation of chondroitin sulphate in embryonic chicken cartilage.
作者信息
Robinson H C
出版信息
Biochem J. 1969 Jul;113(3):543-9. doi: 10.1042/bj1130543.
Abstract
- Whole tissue preparations and subcellular fractions from embryonic chicken cartilage were used to measure the rate of incorporation of inorganic sulphate into chondroitin sulphate in vitro. 2. In cartilage from 14-day-old embryos, [(35)S]sulphate is incorporated to an equal extent into chondroitin 4-sulphate and chondroitin 6-sulphate at a rate of 1.5nmoles of sulphate/hr./mg. dry wt. of cartilage. 3. Microsomal and soluble enzyme preparations from embryonic cartilage catalyse the transfer of sulphate from adenosine 3'-phosphate 5'-sulphatophosphate into both chondroitin 4-sulphate and chondroitin 6-sulphate. 4. The effects of pH, ionic strength, adenosine 3'-phosphate 5'-sulphatophosphate concentration and acceptor chondroitin sulphate concentration on the soluble sulphotransferase activity were examined. These factors all influence the activity of the sulphotransferase, and pH and incubation time also influence the percentage of chondroitin 4-sulphate formed.
摘要
- 利用来自胚胎鸡软骨的全组织制剂和亚细胞组分，在体外测量无机硫酸盐掺入硫酸软骨素的速率。2. 在14日龄胚胎的软骨中，[³⁵S]硫酸盐以1.5纳摩尔硫酸盐/小时/毫克软骨干重的速率等量掺入硫酸软骨素4-硫酸盐和硫酸软骨素6-硫酸盐中。3. 来自胚胎软骨的微粒体和可溶性酶制剂催化硫酸盐从3'-磷酸腺苷5'-硫酸磷酸转移到硫酸软骨素4-硫酸盐和硫酸软骨素6-硫酸盐中。4. 研究了pH、离子强度、3'-磷酸腺苷5'-硫酸磷酸浓度和受体硫酸软骨素浓度对可溶性磺基转移酶活性的影响。这些因素均影响磺基转移酶的活性，并且pH和孵育时间也影响所形成的硫酸软骨素4-硫酸盐的百分比。
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The sulfation of chondroitin sulfate in embryonic chick cartilage epiphyses.胚胎期鸡软骨骨骺中硫酸软骨素的硫酸化作用。
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J Biol Chem. 1968 May 25;243(10):2725-9.
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