D-木糖、β-D-木糖苷和β-D-半乳糖苷对鸡胚软骨中硫酸软骨素生物合成的影响。
The effect of D-xylose, beta-D-xylosides and beta-D-galactosides on chondroitin sulphate biosynthesis in embryonic chicken cartilage.
作者信息
Robinson H C, Brett M J, Tralaggan P J, Lowther D A, Okayama M
出版信息
Biochem J. 1975 Apr;148(1):25-34. doi: 10.1042/bj1480025.
Abstract
The incorporation of [3H]acetate into chondroitin sulphate was used as a measure of the rate of synthesis of this polysaccharide in whole tibias and femurs of embryonic chicken cartilage in vitro. The incorporation is inhibited by puromycin and by cycloheximide, but the inhibition is relieved by the addition of D-xylose, beta-D-xylosides and beta-D-galactosides to the incubation medium. Beta-D-Xylosides can stimulate the incorporation to 300% of that of controls incubated in the absence of cycloheximide or puromycin, D-Xylose, beta-D-xylosides and beta-D-galactosides appear to act as artificial initiators of chondroitin sulphate synthesis and enable polysaccharide-chain synthesis to be studied as an event separate from the synthesis of intact proteoglycan.
摘要
将[3H]乙酸盐掺入硫酸软骨素中，以此作为体外测定胚胎鸡软骨全胫骨和股骨中这种多糖合成速率的一种方法。嘌呤霉素和环己酰亚胺可抑制这种掺入，但向孵育培养基中添加D-木糖、β-D-木糖苷和β-D-半乳糖苷可解除这种抑制。β-D-木糖苷可将掺入量刺激至在无环己酰亚胺或嘌呤霉素的情况下孵育的对照的300%。D-木糖、β-D-木糖苷和β-D-半乳糖苷似乎作为硫酸软骨素合成的人工引发剂，使多糖链合成能够作为一个与完整蛋白聚糖合成分开的事件来研究。
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