金黄色葡萄球菌的磷酸转移酶系统：其对半乳糖苷积累和代谢的需求。
Phosphotransferase system of Staphylococcus aureus: its requirement for the accumulation and metabolism of galactosides.
作者信息
Hengstenberg W, Penberthy W K, Hill K L, Morse M L
出版信息
J Bacteriol. 1969 Aug;99(2):383-8. doi: 10.1128/jb.99.2.383-388.1969.
Abstract
The phosphotransferase system of Staphylococcus aureus was characterized. Mutants defective in enzyme I and heat-stable (HPr) protein as well as in the two components specific to lactose accumulation, factor III and enzyme II, were isolated. Colorimetric assays for each of the components are presented based on the formation of o-nitrophenyl-beta-d-galactoside-6-phosphate by the system and its hydrolysis by the staphylococcal 6-phospho-beta-galactosidase. The components were partially purified and their molecular weights were estimated: enzyme I, 100,000 +/- 15%; HPr, 10,000 +/- 15%; factor III, 30,000 +/- 15%; 6-phospho-beta-galactosidase, 45,000. Enzyme II is a membrane-bound protein.
摘要
对金黄色葡萄球菌的磷酸转移酶系统进行了表征。分离出了在酶I、热稳定（HPr）蛋白以及乳糖积累特异性的两个组分（因子III和酶II）方面存在缺陷的突变体。基于该系统形成邻硝基苯基-β-D-半乳糖苷-6-磷酸并由葡萄球菌6-磷酸-β-半乳糖苷酶将其水解，给出了各组分的比色测定法。对这些组分进行了部分纯化并估计了它们的分子量：酶I为100,000±15%；HPr为10,000±15%；因子III为30,000±15%；6-磷酸-β-半乳糖苷酶为45,000。酶II是一种膜结合蛋白。
相似文献
1
Phosphotransferase system of Staphylococcus aureus: its requirement for the accumulation and metabolism of galactosides.金黄色葡萄球菌的磷酸转移酶系统：其对半乳糖苷积累和代谢的需求。
J Bacteriol. 1969 Aug;99(2):383-8. doi: 10.1128/jb.99.2.383-388.1969.
2
Studies on the mechanism of phosphorylation and transport of beta-galactosides by the lactose phosphotransferase system of Staphylococcus aureus. Kinetic investigations using tosyl galactosides as reversible dead-end inhibitors.金黄色葡萄球菌乳糖磷酸转移酶系统对β-半乳糖苷的磷酸化及转运机制的研究。使用甲苯磺酰半乳糖苷作为可逆性终止抑制剂的动力学研究。
Biochim Biophys Acta. 1976 Aug 16;443(2):267-83. doi: 10.1016/0005-2736(76)90509-5.
3
Mechanisms of lactose utilization by lactic acid streptococci: enzymatic and genetic analyses.乳酸链球菌利用乳糖的机制：酶学与遗传学分析
J Bacteriol. 1970 Jun;102(3):804-9. doi: 10.1128/jb.102.3.804-809.1970.
4
5
Mechanism of hydrolysis of O-nitrophenyl-beta-galactoside in Staphylococcus aureus and its significance for theories of sugar transport.金黄色葡萄球菌中O-硝基苯基-β-半乳糖苷的水解机制及其对糖转运理论的意义。
Proc Natl Acad Sci U S A. 1967 Jul;58(1):225-8. doi: 10.1073/pnas.58.1.225.
6
Characterization of lactose-fermenting revertants from lactose-negative Streptococcus lactis C2 mutants.来自乳糖阴性乳酸乳球菌C2突变体的乳糖发酵回复突变体的特性分析。
J Bacteriol. 1974 Sep;119(3):830-9. doi: 10.1128/jb.119.3.830-839.1974.
7
Photoinactivation of the Staphylococcus aureus Lactose-Specific EIICB Phosphotransferase Component with p-azidophenyl-β-D-Galactoside and Phosphorylation of the Covalently Bound Substrate.对金黄色葡萄球菌乳糖特异性EIICB磷酸转移酶组分进行对叠氮基苯基-β-D-半乳糖苷光灭活及共价结合底物的磷酸化作用
J Mol Microbiol Biotechnol. 2018;28(3):147-158. doi: 10.1159/000494433. Epub 2018 Dec 6.
8
9
Involvement of phosphoenolpyruvate in the catabolism of caries-conducive disaccharides by Streptococcus mutans: lactose transport.磷酸烯醇丙酮酸在变形链球菌对致龋二糖的分解代谢中的作用：乳糖转运
Infect Immun. 1978 Mar;19(3):934-42. doi: 10.1128/iai.19.3.934-942.1978.
10
Studies on the relation of thiomethyl-beta-D-galactoside accumulation to thiomethyl-beta-D-galactoside phosphorylation in Staphylococcus aureus HS1159.金黄色葡萄球菌HS1159中硫代甲基-β-D-半乳糖苷积累与硫代甲基-β-D-半乳糖苷磷酸化关系的研究。
Biochim Biophys Acta. 1968 Oct 15;165(3):410-8. doi: 10.1016/0304-4165(68)90220-1.
引用本文的文献
1
The plasmid-encoded lactose operon plays a vital role in the acid production rate of during milk beverage fermentation.质粒编码的乳糖操纵子在牛奶饮料发酵过程中的产酸率方面起着至关重要的作用。
Front Microbiol. 2022 Oct 6;13:1016904. doi: 10.3389/fmicb.2022.1016904. eCollection 2022.
2
Impact of the Histidine-Containing Phosphocarrier Protein HPr on Carbon Metabolism and Virulence in .含组氨酸的磷酸载体蛋白HPr对[具体对象]碳代谢和毒力的影响
Microorganisms. 2021 Feb 24;9(3):466. doi: 10.3390/microorganisms9030466.
3
Alterations in amino acid metabolism during growth by following exposure to HO - A multifactorial approach.通过后续暴露于HO来研究生长过程中氨基酸代谢的变化——一种多因素方法。
Heliyon. 2018 May 7;4(5):e00620. doi: 10.1016/j.heliyon.2018.e00620. eCollection 2018 May.
4
The bacterial phosphoenolpyruvate:carbohydrate phosphotransferase system: regulation by protein phosphorylation and phosphorylation-dependent protein-protein interactions.细菌磷酸烯醇丙酮酸：碳水化合物磷酸转移酶系统：通过蛋白质磷酸化和磷酸化依赖性蛋白质-蛋白质相互作用进行调控。
Microbiol Mol Biol Rev. 2014 Jun;78(2):231-56. doi: 10.1128/MMBR.00001-14.
5
Induction of Staphylococcus aureus Lactose Permease in the Absence of Glycerolipid Synthesis.在缺乏甘油脂质合成的情况下诱导金黄色葡萄球菌乳糖通透酶
Proc Natl Acad Sci U S A. 1971 Feb;68(2):420-4. doi: 10.1073/pnas.68.2.420.
6
Lactobacillus casei 64H contains a phosphoenolpyruvate-dependent phosphotransferase system for uptake of galactose, as confirmed by analysis of ptsH and different gal mutants.干酪乳杆菌64H含有一个磷酸烯醇式丙酮酸依赖性磷酸转移酶系统用于半乳糖摄取，这通过对ptsH和不同半乳糖突变体的分析得以证实。
J Bacteriol. 1999 Jan;181(1):225-30. doi: 10.1128/JB.181.1.225-230.1999.
7
Cloning and expression of the Listeria monocytogenes scott A ptsH and ptsI genes, coding for HPr and enzyme I, respectively, of the phosphotransferase system.单核细胞增生李斯特菌斯科特A株磷酸转移酶系统中分别编码HPr和酶I的ptsH和ptsI基因的克隆与表达
Appl Environ Microbiol. 1998 Sep;64(9):3147-52. doi: 10.1128/AEM.64.9.3147-3152.1998.
8
Catabolite repression resistance of gnt operon expression in Bacillus subtilis conferred by mutation of His-15, the site of phosphoenolpyruvate-dependent phosphorylation of the phosphocarrier protein HPr.枯草芽孢杆菌中gnt操纵子表达的分解代谢物阻遏抗性由His-15突变赋予，His-15是磷酸载体蛋白HPr的磷酸烯醇丙酮酸依赖性磷酸化位点。
J Bacteriol. 1996 Sep;178(18):5480-6. doi: 10.1128/jb.178.18.5480-5486.1996.
9
Presence of protein constituents of the gram-positive bacterial phosphotransferase regulatory system in Acholeplasma laidlawii.莱氏无胆甾原体中革兰氏阳性菌磷酸转移酶调节系统蛋白质成分的存在。
J Bacteriol. 1993 Oct;175(20):6599-604. doi: 10.1128/jb.175.20.6599-6604.1993.
10
Properties of ATP-dependent protein kinase from Streptococcus pyogenes that phosphorylates a seryl residue in HPr, a phosphocarrier protein of the phosphotransferase system.化脓性链球菌中能使磷酸转移酶系统的磷酸载体蛋白HPr中的一个丝氨酸残基磷酸化的ATP依赖性蛋白激酶的特性。
J Bacteriol. 1984 Oct;160(1):333-40. doi: 10.1128/jb.160.1.333-340.1984.
本文引用的文献
1
TRANSDUCTION BY STAPHYLOCOCCAL BACTERIOPHAGE.葡萄球菌噬菌体转导
Proc Natl Acad Sci U S A. 1959 May;45(5):722-7. doi: 10.1073/pnas.45.5.722.
2
CARBOHYDRATE TRANSPORT IN STAPHYLOCOCCUS AUREUS I. GENETIC AND BIOCHEMICAL ANALYSIS OF A PLEIOTROPIC TRANSPORT MUTANT.金黄色葡萄球菌中的碳水化合物转运 I. 一个多效性转运突变体的遗传与生化分析
Biochim Biophys Acta. 1965 Feb 15;97:310-9. doi: 10.1016/0304-4165(65)90096-6.
3
PHOSPHATE BOUND TO HISTIDINE IN A PROTEIN AS AN INTERMEDIATE IN A NOVEL PHOSPHO-TRANSFERASE SYSTEM.蛋白质中与组氨酸结合的磷酸盐作为新型磷转移酶系统中的中间体。
Proc Natl Acad Sci U S A. 1964 Oct;52(4):1067-74. doi: 10.1073/pnas.52.4.1067.
4
An agar medium indicating acid production.一种指示产酸的琼脂培养基。
J Bacteriol. 1958 Sep;76(3):270-1. doi: 10.1128/jb.76.3.270-271.1958.
5
Carbohydrate transport in Staphylococcus aureus. II. Characterization of the defect of a pleiotropic transport mutant.金黄色葡萄球菌中的碳水化合物转运。II. 多效性转运突变体缺陷的特征描述。
Biochim Biophys Acta. 1965 Sep 27;109(1):172-83. doi: 10.1016/0926-6585(65)90101-9.
6
The gel-filtration behaviour of proteins related to their molecular weights over a wide range.蛋白质的凝胶过滤行为与其在很宽范围内的分子量相关。
Biochem J. 1965 Sep;96(3):595-606. doi: 10.1042/bj0960595.
7
Studies on the relation of thiomethyl-beta-D-galactoside accumulation to thiomethyl-beta-D-galactoside phosphorylation in Staphylococcus aureus HS1159.金黄色葡萄球菌HS1159中硫代甲基-β-D-半乳糖苷积累与硫代甲基-β-D-半乳糖苷磷酸化关系的研究。
Biochim Biophys Acta. 1968 Oct 15;165(3):410-8. doi: 10.1016/0304-4165(68)90220-1.
8
Metabolism of lactose by Staphylococcus aureus.金黄色葡萄球菌对乳糖的代谢
J Bacteriol. 1968 Dec;96(6):2187-8. doi: 10.1128/jb.96.6.2187-2188.1968.
9
Metabolism of lactose by Staphylococcus aureus and its genetic basis.金黄色葡萄球菌对乳糖的代谢及其遗传基础。
J Bacteriol. 1968 Jun;95(6):2270-4. doi: 10.1128/jb.95.6.2270-2274.1968.
10
Resolution of a staphylococcal phosphotransferase system into four protein components and its relation to sugar transport.葡萄球菌磷酸转移酶系统分解为四种蛋白质成分及其与糖转运的关系。
Biochem Biophys Res Commun. 1968 Jun 10;31(5):804-11. doi: 10.1016/0006-291x(68)90634-7.
Zotero 插件