单核细胞增生李斯特菌斯科特A株磷酸转移酶系统中分别编码HPr和酶I的ptsH和ptsI基因的克隆与表达
Cloning and expression of the Listeria monocytogenes scott A ptsH and ptsI genes, coding for HPr and enzyme I, respectively, of the phosphotransferase system.
作者信息
Christensen D P, Benson A K, Hutkins R W
机构信息
School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska 68583-0919, USA.
出版信息
Appl Environ Microbiol. 1998 Sep;64(9):3147-52. doi: 10.1128/AEM.64.9.3147-3152.1998.
Abstract
The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) utilizes high-energy phosphate present in PEP to drive the uptake of several different carbohydrates in bacteria. In order to examine the role of the PTS in the physiology of Listeria monocytogenes, we identified the ptsH and ptsI genes encoding the HPr and enzyme I proteins, respectively, of the PTS. Nucleotide sequence analysis indicated that the predicted proteins are nearly 70% similar to HPr and enzyme I proteins from other organisms. Purified L. monocytogenes HPr overexpressed in Escherichia coli was also capable of complementing an HPr defect in heterologous extracts of Staphylococcus aureus ptsH mutants. Additional studies of the transcriptional organization and control indicated that the ptsH and ptsI genes are organized into a transcription unit that is under the control of a consensus-like promoter and that expression of these genes is mediated by glucose availability and pH or by by-products of glucose metabolism.
摘要
磷酸烯醇丙酮酸（PEP）依赖性磷酸转移酶系统（PTS）利用PEP中存在的高能磷酸基团来驱动细菌摄取几种不同的碳水化合物。为了研究PTS在单核细胞增生李斯特菌生理学中的作用，我们分别鉴定了编码PTS的HPr和酶I蛋白的ptsH和ptsI基因。核苷酸序列分析表明，预测的蛋白质与来自其他生物体的HPr和酶I蛋白相似度近70%。在大肠杆菌中过表达的纯化单核细胞增生李斯特菌HPr也能够弥补金黄色葡萄球菌ptsH突变体异源提取物中的HPr缺陷。对转录组织和调控的进一步研究表明，ptsH和ptsI基因组成一个转录单元，该转录单元受一个类似共有序列的启动子控制，并且这些基因的表达由葡萄糖可用性、pH值或葡萄糖代谢副产物介导。
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