Storck R, Alexopoulos C J, Phaff H J
J Bacteriol. 1969 Jun;98(3):1069-72. doi: 10.1128/jb.98.3.1069-1072.1969.
The buoyant density of deoxyribonucleic acid (DNA) from nine species and two varieties of Cryptococcus, three species and two varieties of Rhodotorula, and six species of Sporobolomyces was determined by CsCl density gradient equilibrium centrifugation. Several species were represented by two to four different strains. Expressed in moles per cent of guanine plus cytosine (GC content) the ranges were 49 to 65%, 52 to 70%, and 51 to 65% for Cryptococcus, Rhodotorula, and Sporobolomyces, respectively. For each genus, the GC content was distributed into two discrete groups with averages ranging from 52 to 54 and 60 to 66, respectively. An analysis of these results suggested that the determination of GC content of DNA had a taxonomic value for these yeast genera.
通过氯化铯密度梯度平衡离心法测定了来自9个种和2个变种的隐球菌、3个种和2个变种的红酵母以及6个种的掷孢酵母的脱氧核糖核酸(DNA)的浮力密度。几个种由2至4个不同菌株代表。以鸟嘌呤加胞嘧啶的摩尔百分比(GC含量)表示,隐球菌、红酵母和掷孢酵母的范围分别为49%至65%、52%至70%和51%至65%。对于每个属,GC含量分布在两个离散的组中,平均值分别为52%至54%和60%至66%。对这些结果的分析表明,DNA的GC含量测定对这些酵母属具有分类学价值。