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1
Characteristics of beta-galactosidase in the mucosa of the small intestine of infant rats. Physicochemical properties.幼鼠小肠黏膜中β-半乳糖苷酶的特性。物理化学性质。
Biochem J. 1969 Sep;114(3):621-7. doi: 10.1042/bj1140621.
2
Effect of cortisone on the developmental pattern of the neutral and the acid beta-galactosidase of the small intestine of the rat.可的松对大鼠小肠中性和酸性β-半乳糖苷酶发育模式的影响。
Biochem J. 1970 Apr;117(3):467-71. doi: 10.1042/bj1170467.
3
Small intestinal beta-galactosidase activity in the horse.马的小肠β-半乳糖苷酶活性
Gut. 1973 Jul;14(7):535-40. doi: 10.1136/gut.14.7.535.
4
[On the beta-galactosidase activity of the rat small intestine].[关于大鼠小肠的β-半乳糖苷酶活性]
Acta Biol Med Ger. 1968;20(4):421-6.
5
-Glucosidase (phlorizin hydrolase) activity of the lactase fraction isolated from the small intestinal mucosa of infant rats, and the relationship between -glucosidases and -galactosidases.从幼鼠小肠黏膜分离的乳糖酶组分的β-葡萄糖苷酶（根皮苷水解酶）活性，以及β-葡萄糖苷酶与β-半乳糖苷酶之间的关系。
Biochim Biophys Acta. 1972 Feb 28;258(2):520-30. doi: 10.1016/0005-2744(72)90243-4.
6
Human small-intestinal -galactosidases. Separation and characterization of three forms of an acid -galactosidase.人小肠β-半乳糖苷酶。一种酸性β-半乳糖苷酶三种形式的分离与特性鉴定
Biochem J. 1971 Jan;121(2):299-308. doi: 10.1042/bj1210299.
7
Human small-intestinal beta-galactosidases. Separation and characterization of one lactase and one hetero beta-galactosidase.人类小肠β-半乳糖苷酶。一种乳糖酶和一种杂合β-半乳糖苷酶的分离与特性分析。
Biochem J. 1969 Sep;114(2):351-9. doi: 10.1042/bj1140351.
8
Rat small-intestinal beta-galactosidases. Separation by ion-exchange chromatography and gel filtration.大鼠小肠β-半乳糖苷酶。通过离子交换色谱法和凝胶过滤法进行分离。
Biochem J. 1968 Feb;106(4):841-5. doi: 10.1042/bj1060841.
9
Comparison of activities of acid -galactosidase, -glucuronidase, N-acetyl- -glucosaminidase and -galactosidase in jejunum and ileum of adult and suckling rats.
Comp Biochem Physiol B. 1972 Sep 15;43(1):1-8. doi: 10.1016/0305-0491(72)90193-9.
10
Lactase enzymes in the intestinal brush border membrane of the suckling rat. A second enzyme restricted to ileum.
FEBS Lett. 1977 Dec 15;84(2):233-5. doi: 10.1016/0014-5793(77)80695-9.

引用本文的文献

1
Rat small-intestinal beta-galactosidases. Studies on the fractionation of "acid" beta-galactosidase with isoelectric focusing, gel filtration and ion-exchange chromatography.大鼠小肠β-半乳糖苷酶。关于用等电聚焦、凝胶过滤和离子交换色谱法分离“酸性”β-半乳糖苷酶的研究。
Biochem J. 1970 Apr;117(2):369-75. doi: 10.1042/bj1170369.
2
Effect of cortisone on the developmental pattern of the neutral and the acid beta-galactosidase of the small intestine of the rat.可的松对大鼠小肠中性和酸性β-半乳糖苷酶发育模式的影响。
Biochem J. 1970 Apr;117(3):467-71. doi: 10.1042/bj1170467.
3
Human small-intestinal -galactosidases. Separation and characterization of three forms of an acid -galactosidase.人小肠β-半乳糖苷酶。一种酸性β-半乳糖苷酶三种形式的分离与特性鉴定
Biochem J. 1971 Jan;121(2):299-308. doi: 10.1042/bj1210299.
4
Effects of proteins and polynucleotides on the activity of various hydrolases.蛋白质和多核苷酸对各种水解酶活性的影响。
Biochem J. 1972 May;127(5):795-800. doi: 10.1042/bj1270795.
5
Indigogenic methods for glycosidases. 3. An improved method with 4-Cl-5-Br-3-indolyl-beta-D-fucoside and its application in studies of enzymes in the intestine, kidney and other tissues.糖苷酶的产靛方法。3. 一种使用4-氯-5-溴-3-吲哚基-β-D-岩藻糖苷的改进方法及其在肠道、肾脏和其他组织中酶研究中的应用。
Histochemie. 1971;25(3):195-207. doi: 10.1007/BF00305938.
6
Indigogenic methods for glycosidases. II. An improved method for beta-D-galactosidase and its application to localization studies of the enzymes in the intestine and in other tissues.糖苷酶的产靛方法。II. β-D-半乳糖苷酶的一种改进方法及其在肠道和其他组织中酶定位研究的应用。
Histochemie. 1970;23(3):266-88. doi: 10.1007/BF00306428.
7
Indigogenic methods for glycosidases. I. An improved method for beta-D-glucosidase and its application to localization studies on intestinal and renal enzymes.糖苷酶的靛蓝生成法。I. β-D-葡萄糖苷酶的一种改进方法及其在肠道和肾脏酶定位研究中的应用。
Histochemie. 1970;22(4):347-61. doi: 10.1007/BF00277462.

本文引用的文献

1
Specificity and multiple forms of beta-galactosidase in the rat.大鼠体内β-半乳糖苷酶的特异性及多种形式
Biochem J. 1965 Oct;97(1):59-66. doi: 10.1042/bj0970059.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Inhibition of glycosidases by aldonolactones of corresponding configuration. 3. Inhibitors of beta-D-galactosidase.相应构型的醛糖内酯对糖苷酶的抑制作用。3. β-D-半乳糖苷酶抑制剂。
Biochem J. 1962 Feb;82(2):225-32. doi: 10.1042/bj0820225.
4
POSTNATAL DEVELOPMENT OF BETA-GALACTOSIDASE ACTIVITY IN THE SMALL INTESTINE OF THE RAT. EFFECT OF ADRENALECTOMY AND DIET.大鼠小肠中β-半乳糖苷酶活性的产后发育。肾上腺切除术和饮食的影响。
Biochem J. 1965 Jan;94(1):266-70. doi: 10.1042/bj0940266.
5
CONCERNING THE TRANSPORT OF LACTOSE ACROSS THE INTESTINAL WALL OF INFANT RATS.关于乳糖在新生大鼠肠壁中的转运
Can J Physiol Pharmacol. 1965 May;43:469-71.
6
MULTIPLICITY OF HUMAN INTESTINAL DISACCHARIDASES. I. CHROMATOGRAPHIC SEPARATION OF MALTASES AND OF TWO LACTASES.人肠道双糖酶的多样性。I. 麦芽糖酶和两种乳糖酶的色谱分离
Biochim Biophys Acta. 1965 Mar 22;96:487-97. doi: 10.1016/0005-2787(65)90565-4.
7
Studies of small intestine during development. II. The intracellular location of intestinal beta-galactosidase.发育过程中小肠的研究。II. 肠道β-半乳糖苷酶的细胞内定位。
Biochim Biophys Acta. 1963 Mar 12;67:516-9. doi: 10.1016/0006-3002(63)91860-2.
8
Studies of small intestine during development. I. Distribution and activity of beta-galactosidase.发育过程中小肠的研究。I. β-半乳糖苷酶的分布与活性。
Biochim Biophys Acta. 1962 Aug 13;62:353-62. doi: 10.1016/0006-3002(62)90097-5.
9
Rat small-intestinal beta-galactosidases. Influence of pH on the hydrolysis of different substrates.大鼠小肠β-半乳糖苷酶。pH对不同底物水解的影响。
Biochem J. 1967 Apr;103(1):86-9. doi: 10.1042/bj1030086.
10
Beta-galactosidase activity of the jejunum and ileum of suckling rats. Comparison of activities of beta-galactosidase at different concentration of substrates (o-nitrophenyl-beta-D-galactoside and lactose) at different pH.哺乳期大鼠空肠和回肠的β-半乳糖苷酶活性。不同pH值下不同底物浓度（邻硝基苯基-β-D-半乳糖苷和乳糖）时β-半乳糖苷酶活性的比较。
Biol Neonat. 1966;10(5):241-53.

幼鼠小肠黏膜中β-半乳糖苷酶的特性。物理化学性质。

Characteristics of beta-galactosidase in the mucosa of the small intestine of infant rats. Physicochemical properties.

作者信息

Kraml J, Koldovský O, Heringová A, Jirsová V, Kácl K, Ledvina M, Pelichová H

出版信息

Biochem J. 1969 Sep;114(3):621-7. doi: 10.1042/bj1140621.

DOI:10.1042/bj1140621
PMID:5820646
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1184935/
Abstract
  1. The characteristics of acid and neutral beta-galactosidases isolated chromatographically from homogenates of the mucosa of the jejunum and ileum of suckling rats were studied. 2. The minimal molecular weight of the acid beta-galactosidase, as estimated by gel filtration on Sephadex G-200, was in the range 83000-105000, whereas for the neutral beta-galactosidase the estimated molecular weight was in the range 360000-510000. 3. The acid and neutral beta-galactosidases were inhibited competitively by galactono-(1-->4)-lactone, with respective K(i) values of 0.15mm and 1.1mm. Only the acid beta-galactosidase was inhibited competitively by sodium galactonate (K(i) 0.17mm). 4. Heat inactivation of both beta-galactosidases occurred according to first-order kinetics. The neutral enzyme was more labile, but bovine serum albumin protected acid enzyme only. 5. Urea treatment inactivated both beta-galactosidases, the neutral beta-galactosidase being more sensitive than the acid beta-galactosidase. 6. No differences were found between preparations from the jejunum and ileum.
摘要
  1. 对从乳鼠空肠和回肠黏膜匀浆中通过色谱法分离得到的酸性和中性β-半乳糖苷酶的特性进行了研究。2. 通过在Sephadex G - 200上进行凝胶过滤估计，酸性β-半乳糖苷酶的最小分子量在83000 - 105000范围内，而中性β-半乳糖苷酶的估计分子量在360000 - 510000范围内。3. 半乳糖酸内酯（1→4）-内酯对酸性和中性β-半乳糖苷酶有竞争性抑制作用，其各自的K(i)值分别为0.15mmol/L和1.1mmol/L。只有酸性β-半乳糖苷酶受到半乳糖酸钠的竞争性抑制（K(i) 0.17mmol/L）。4. 两种β-半乳糖苷酶的热失活均符合一级动力学。中性酶更不稳定，但牛血清白蛋白仅对酸性酶有保护作用。5. 尿素处理使两种β-半乳糖苷酶失活，中性β-半乳糖苷酶比酸性β-半乳糖苷酶更敏感。6. 在空肠和回肠的制剂之间未发现差异。