幼鼠小肠黏膜中β-半乳糖苷酶的特性。物理化学性质。
Characteristics of beta-galactosidase in the mucosa of the small intestine of infant rats. Physicochemical properties.
作者信息
Kraml J, Koldovský O, Heringová A, Jirsová V, Kácl K, Ledvina M, Pelichová H
出版信息
Biochem J. 1969 Sep;114(3):621-7. doi: 10.1042/bj1140621.
Abstract
- The characteristics of acid and neutral beta-galactosidases isolated chromatographically from homogenates of the mucosa of the jejunum and ileum of suckling rats were studied. 2. The minimal molecular weight of the acid beta-galactosidase, as estimated by gel filtration on Sephadex G-200, was in the range 83000-105000, whereas for the neutral beta-galactosidase the estimated molecular weight was in the range 360000-510000. 3. The acid and neutral beta-galactosidases were inhibited competitively by galactono-(1-->4)-lactone, with respective K(i) values of 0.15mm and 1.1mm. Only the acid beta-galactosidase was inhibited competitively by sodium galactonate (K(i) 0.17mm). 4. Heat inactivation of both beta-galactosidases occurred according to first-order kinetics. The neutral enzyme was more labile, but bovine serum albumin protected acid enzyme only. 5. Urea treatment inactivated both beta-galactosidases, the neutral beta-galactosidase being more sensitive than the acid beta-galactosidase. 6. No differences were found between preparations from the jejunum and ileum.
摘要
- 对从乳鼠空肠和回肠黏膜匀浆中通过色谱法分离得到的酸性和中性β-半乳糖苷酶的特性进行了研究。2. 通过在Sephadex G - 200上进行凝胶过滤估计，酸性β-半乳糖苷酶的最小分子量在83000 - 105000范围内，而中性β-半乳糖苷酶的估计分子量在360000 - 510000范围内。3. 半乳糖酸内酯（1→4）-内酯对酸性和中性β-半乳糖苷酶有竞争性抑制作用，其各自的K(i)值分别为0.15mmol/L和1.1mmol/L。只有酸性β-半乳糖苷酶受到半乳糖酸钠的竞争性抑制（K(i) 0.17mmol/L）。4. 两种β-半乳糖苷酶的热失活均符合一级动力学。中性酶更不稳定，但牛血清白蛋白仅对酸性酶有保护作用。5. 尿素处理使两种β-半乳糖苷酶失活，中性β-半乳糖苷酶比酸性β-半乳糖苷酶更敏感。6. 在空肠和回肠的制剂之间未发现差异。
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CONCERNING THE TRANSPORT OF LACTOSE ACROSS THE INTESTINAL WALL OF INFANT RATS.关于乳糖在新生大鼠肠壁中的转运
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