Merritt G C, Watts J E
Aust Vet J. 1978 Nov;54(11):513-6. doi: 10.1111/j.1751-0813.1978.tb00317.x.
Fleece-rot was experimentally induced in-vitro by wetting and incubating Merino wool samples embedded in serum-agar. Gravid Lucilia cuprina were readily-attracted to these wool culture plates to oviposit. Where serum was freely available to newly-hatched larvae, fly strike and larval development ensued. Using this technique, fleece-rot and oviposition were found to be markedly influenced by the availability of protein and by bacterial activity, particularly that of Pseudomonas spp. The results indicated that odours emanating from wool culture plates containing the latter species played an important role in oviposition. Furthermore, these events varied according to the type of fleece selected, and could be prevented by the addition of a bactericide.
通过将埋于血清琼脂中的美利奴羊毛样本浸湿并培养，在体外实验诱导了羊毛腐病。怀孕的铜绿蝇很容易被这些羊毛培养平板吸引来产卵。当新孵化的幼虫能够自由获取血清时，就会发生蝇蛆侵袭和幼虫发育。利用这项技术，发现羊毛腐病和产卵受到蛋白质可用性和细菌活性的显著影响，尤其是假单胞菌属细菌的活性。结果表明，含有后一种细菌的羊毛培养平板散发的气味在产卵过程中起重要作用。此外，这些情况会因所选羊毛类型而异，并且可以通过添加杀菌剂来预防。
