London C J, Griffith I P, Kortt A A
Appl Environ Microbiol. 1984 Jan;47(1):75-9. doi: 10.1128/aem.47.1.75-79.1984.
Fifty-nine pseudomonads isolated from sheep fleece were able to grow on a minimal salts medium with glycerol as the sole source of carbon and energy. Many of these isolates showed additional growth when collagen-based or wool-based substrates were included in the medium. After several days of incubation with these substrates, the nature of soluble proteins present in the growth medium was investigated by using polyacrylamide gel electrophoresis. Up to four major bands of protein with proteinase activity and with widely different electrophoretic mobilities were detected in the gels; one of the bands appeared as a doublet at times. The electrophoretic mobilities of each class of proteinase were similar for the different pseudomonads examined, but the proteinase (or combination of proteinases) induced depended on the protein substrate and strain or species of pseudomonad used.
从羊毛中分离出的59株假单胞菌能够在以甘油作为唯一碳源和能源的基本盐培养基上生长。当培养基中加入基于胶原蛋白或羊毛的底物时，许多这些分离株表现出额外的生长。在与这些底物孵育几天后，通过聚丙烯酰胺凝胶电泳研究生长培养基中存在的可溶性蛋白质的性质。在凝胶中检测到多达四条具有蛋白酶活性且电泳迁移率差异很大的主要蛋白带；其中一条带有时呈现为双峰。对于所检测的不同假单胞菌，每类蛋白酶的电泳迁移率相似，但诱导产生的蛋白酶（或蛋白酶组合）取决于蛋白质底物以及所用假单胞菌的菌株或种类。
