Nardone R M, Todd J, Gonzalez P, Gaffney E V
Science. 1965 Sep 3;149(3688):1100-1. doi: 10.1126/science.149.3688.1100.
Contamination of Strain L cell cultures by pleuropneumonia-like organismtis (PPLO) resulted in a complete inhibition of the incorporation of tritiated thymnidine and uridine. Contanminated cultures were characterized by a concentration of PPLO along the margins of the cells and in the intercellular-spaces of the cultures. Autoracliograins of PPLO-contaminated cultures were characterized by exposed silver grains over the margins of the cells. Kanamnycin was efjective in the elimination of PPLO and the restoration of nucleoside incorporation.
类胸膜肺炎微生物（PPLO）污染L细胞培养物导致氚标记胸腺嘧啶核苷和尿苷的掺入完全受到抑制。受污染的培养物的特征是PPLO集中在细胞边缘和培养物的细胞间隙中。PPLO污染培养物的放射自显影片的特征是细胞边缘有银颗粒曝光。卡那霉素在消除PPLO和恢复核苷掺入方面有效。
