Berg H C, Diamond J M, Marfey P S
Science. 1965 Oct 1;150(3692):64-7. doi: 10.1126/science.150.3692.64.
Erythrocytes treated with 1-fluoro-2,4-dinitrobenzene become permeable to Na(+) and K(+), but not to small water-soluble nonelectrolytes hemoglobin, and eventually lyse inisotonic buffer. Erythrocytes treated with 1,5-difluoro-2,4-dinitrobenzene become permeable to Na(+) and K(+) but do not lyse in buffer or in water, even after extraction with lipid solvents. The difluoro compound crosslinks the membrane and increases its strength. Both reagents appear to remove the positive fixed charge responsible for the cation impermeability of the normal cell.
用1-氟-2,4-二硝基苯处理的红细胞对Na(+)和K(+)具有通透性，但对小分子水溶性非电解质血红蛋白不通透，最终在等渗缓冲液中裂解。用1,5-二氟-2,4-二硝基苯处理的红细胞对Na(+)和K(+)具有通透性，但即使在用脂质溶剂提取后，在缓冲液或水中也不会裂解。二氟化合物使膜交联并增加其强度。两种试剂似乎都去除了负责正常细胞阳离子不可渗透性的正固定电荷。
