Varma R
Biochem Exp Biol. 1978;14(4):311-8.
A simple, sensitive and precise gas-chromatographic method for simultaneous extraction, derivatization and determination of methsuximide, ethosuximide, diphenylhydantoin, carbamazepine, phenobarbital and primidone in the presence of other drugs has been described. The method is especially useful for drug monitoring in patients on multiple anticonvulsant therapy while also on combination therapy with psychotropic drugs. It overcomes the analytical interferences between mephenytoin and phenobarbital; methsuximide and primidone; kemadrin and primidone; cholesterol and primidone; prolixin, haldol and other drugs; encountered in other methods using underivatized, trimethylsilylated or methylated drugs. As little as 0.5 microgram/ml of a drug can be determined and if needed the method can be scaled down to 0.3 ml plasma. The method yielded recoveries of 97-103% with standard deviations of 0.7-1.8. For a constant check of the precision, an internal quality control using daily analysis of a sample from a frozen plasma pool supplemented with known concentrations of the anticonvulsants was used. The method is suitable for use in routine clinical laboratory.
本文描述了一种简单、灵敏且精确的气相色谱法，可在存在其他药物的情况下，同时提取、衍生化并测定甲琥胺、乙琥胺、苯妥英、卡马西平、苯巴比妥和扑米酮。该方法对于接受多种抗惊厥药物治疗且同时接受精神药物联合治疗的患者的药物监测特别有用。它克服了其他使用未衍生化、三甲基硅烷化或甲基化药物的方法中所遇到的美芬妥英与苯巴比妥、甲琥胺与扑米酮、开马君与扑米酮、胆固醇与扑米酮、丙氯拉嗪、氟哌啶醇及其他药物之间的分析干扰。该方法能够测定低至0.5微克/毫升的药物，如有需要，可将方法规模缩小至0.3毫升血浆。该方法的回收率为97 - 103%，标准偏差为0.7 - 1.8。为持续检查精密度，采用了内部质量控制，即每日分析来自添加了已知浓度抗惊厥药物的冷冻血浆库的样本。该方法适用于常规临床实验室。
