核糖体：通过硫酸铯梯度离心法进行分析
Ribosomes: analysis by cesium sulfate gradient centrifugation.
作者信息
DeFilippes F M
出版信息
Science. 1965 Oct 29;150(3696):610-2. doi: 10.1126/science.150.3696.610.
PMID:5837097
Abstract
Ribosomes from the cytoplasm of a mammalian tissue, HeLa cells, were partially purified and then concentrated into a zone by prolonged centrifugation in Cs(2)SO(4) solution (density, 1.43 grams per milliliter). Ribosomal preparations treated with bentonite prior to density-gradient centrifugation showed single peaks after centrifugation. Material recovered from the peaks represented aggregates of the original 74S particle.
摘要
从哺乳动物组织（HeLa细胞）的细胞质中提取的核糖体被部分纯化，然后通过在硫酸铯溶液（密度为1.43克/毫升）中长时间离心浓缩成一个区带。在进行密度梯度离心之前用膨润土处理的核糖体制剂在离心后显示出单峰。从峰中回收的物质代表原始74S颗粒的聚集体。
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