Kolehmainen L, Zech H, von Wettstein D
J Cell Biol. 1965 Jun;25(3):Suppl:77-97. doi: 10.1083/jcb.25.3.77.
The submicroscopic organization of mesophyll cells from tobacco leaves systemically infected with tobacco mosaic virus (TMV) is described. After fixation with glutaraldehyde and osmium tetroxide the arrangement of the TMV particles within the crystalline inclusions is well preserved. Only the ribonucleic acid-containing core of the virus particles is visible in the micrographs. Besides the hexagonal virus crystals, several characteristic types of "inclusion bodies" are definable in the cytoplasm: The so-called fluid crystals seem to correspond to single layers of oriented TMV particles between a network of the endoplasmic reticulum and ribosomes. Unordered groups or well oriented masses of tubes with the diameter of the TMV capsid are found in certain areas of the cytoplasm. A complicated inclusion body is characterized by an extensively branched and folded part of the endoplasmic reticulum, containing in its folds long aggregates of flexible rods. Certain parts of the cytoplasm are filled with large, strongly electron-scattering globules, probably of lipid composition. These various cytoplasmic differentiations and the different forms of presumed virus material are discussed in relation to late stages of TMV reproduction and virus crystal formation.
本文描述了被烟草花叶病毒（TMV）系统感染的烟草叶片中叶肉细胞的亚微观结构。用戊二醛和四氧化锇固定后，结晶内含物中TMV颗粒的排列得以良好保存。在显微照片中只能看到病毒颗粒含核糖核酸的核心。除了六边形病毒晶体外，在细胞质中还可确定几种特征性的“包涵体”类型：所谓的流体晶体似乎对应于内质网和核糖体网络之间单层排列的定向TMV颗粒。在细胞质的某些区域发现了直径与TMV衣壳相同的无序管群或排列良好的管团。一种复杂的包涵体的特征是内质网有广泛分支和折叠的部分，其褶皱中含有长的柔性杆状聚集体。细胞质的某些部分充满了大的、电子散射强烈的小球，可能由脂质组成。结合TMV复制后期和病毒晶体形成，讨论了这些不同的细胞质分化以及假定病毒物质的不同形式。
