Forer A
J Cell Sci. 1975 Dec;19(3):579-605. doi: 10.1242/jcs.19.3.579.
Microtubules in crane-fly spermatids appeared altered when the glutaraldehyde-fixed cells were not postfixed with osmium tetroxide. The cytoplasmic microtubules were altered more than the doublet microtubules. Addition of osmium tetroxide after dehydration did not produce appearances identical with those of microtubules postfixed directly after glutaraldehyde, and thus at least some alterations occurred during dehydration, possibly due to extraction of microtubule-associated lipid. The omission of osmium tetroxide postfixation did not cause drastic alterations in the appearances of either tobacco mosaic virus (TMV), or polymerized tobacco mosaic virus protein (without RNA), suggesting that microtubule stability is different from TMV stability (with respect to the embedment procedure). The electron-dense stain associated with embedded-sectioned TMV is predominantly outside the TMV protein, as demonstrated by the known distribution of TMV protein compared with the dimensions of sectioned TMV and negatively stained TMV. The same might hold true for microtubules, as evidenced by the dimensions of negatively stained, isolated brain microtubules compared with those of embedded and sectioned brain microtubules.
当戊二醛固定的大蚊精子细胞未用四氧化锇进行后固定时，其微管会出现变化。细胞质微管的变化比双联微管更明显。脱水后添加四氧化锇所产生的外观与戊二醛固定后直接进行后固定的微管外观并不相同，因此至少有一些变化发生在脱水过程中，这可能是由于与微管相关的脂质被提取所致。省略四氧化锇后固定并未导致烟草花叶病毒（TMV）或聚合的烟草花叶病毒蛋白（无RNA）的外观发生剧烈变化，这表明微管稳定性与TMV稳定性（就包埋程序而言）不同。与包埋切片的TMV相关的电子致密染色主要在TMV蛋白之外，这是通过将已知的TMV蛋白分布与切片TMV和负染TMV的尺寸进行比较来证明的。微管可能也是如此，这可通过将负染的分离脑微管的尺寸与包埋和切片的脑微管的尺寸进行比较来证明。
