Gibson K D
J Bacteriol. 1965 Oct;90(4):1059-72. doi: 10.1128/jb.90.4.1059-1072.1965.
Gibson, K. D. (St. Mary's Hospital Medical School, London, England). Electron microscopy of Rhodopseudomonas spheroides. J. Bacteriol. 90:1059-1072. 1965.-Fixed and stained chromatophores and whole cells of anaerobically grown Rhodopseudomonas spheroides were examined in thin sections in the electron microscope. Both purified chromatophores and intracellular membrane-bound vesicles had exactly the same appearance, namely that of spheres or ellipsoids with a thin electron-dense shell surrounding an electron-lucent interior. The distribution of diameters in the two types of structure was also found to be the same, and was compatible with a normal distribution, with a mean of 570 A and a standard deviation 40 A. Negatively stained chromatophores appeared like discs or collapsed spheres. The presence of invaginations of the cytoplasmic membrane in this species was confirmed, and a new structure resembling a twin chromatophore was observed. The bearing of these results on theories of the origin of chromatophores is discussed, and it is concluded that they offer some support for each one of the three main theories about the origin of particulate organelles.
吉布森，K. D.（英国伦敦圣玛丽医院医学院）。球形红假单胞菌的电子显微镜观察。《细菌学杂志》90:1059 - 1072。1965年。——对厌氧培养的球形红假单胞菌的固定、染色后的载色体和完整细胞进行了超薄切片的电子显微镜观察。纯化的载色体和细胞内膜结合小泡外观完全相同，即呈球体或椭球体，有一层薄的电子致密外壳包围着电子透明的内部。还发现这两种结构的直径分布相同，符合正态分布，平均值为570埃，标准差为40埃。经负染色的载色体呈圆盘状或塌陷的球体状。证实了该菌种中存在细胞质膜内陷现象，并观察到一种类似双载色体的新结构。讨论了这些结果对载色体起源理论的影响，得出结论：它们为关于颗粒细胞器起源的三种主要理论中的每一种都提供了一些支持。
