Briggs F N, Fleishman M
J Gen Physiol. 1965 Sep;49(1):131-49.
A high molecular weight fraction of a soluble Marsh muscle-relaxing preparation has been shown to contain a calcium-complexing substance. By examining the nature of the competition between this fraction and chelex-100 for calcium at various total calcium concentrations it has been possible to calculate the concentration and calcium stability constant of this calcium-complexing substance. Taking into account dilutions which occur during the preparation of fractions containing this substance its concentration may be estimated at about 2.10(-4) in muscle and its calcium stability constant was found to be about 1.5.10(5)M(-1). Preliminary evidence suggests that the calcium-binding substance is a protein.
一种可溶性马什肌肉松弛制剂的高分子量组分已被证明含有一种钙络合物质。通过研究该组分与螯合树脂100在不同总钙浓度下对钙的竞争性质，可以计算出这种钙络合物质的浓度和钙稳定性常数。考虑到在制备含有该物质的组分过程中发生的稀释情况，其在肌肉中的浓度估计约为2×10⁻⁴，其钙稳定性常数约为1.5×10⁵M⁻¹。初步证据表明，钙结合物质是一种蛋白质。
