骨骼肌匀浆无颗粒上清液与钙的结合
Calcium binding by particle-free supernatants of homogenates of skeletal muscle.
作者信息
Briggs F N, Fleishman M
出版信息
J Gen Physiol. 1965 Sep;49(1):131-49.
Abstract
A high molecular weight fraction of a soluble Marsh muscle-relaxing preparation has been shown to contain a calcium-complexing substance. By examining the nature of the competition between this fraction and chelex-100 for calcium at various total calcium concentrations it has been possible to calculate the concentration and calcium stability constant of this calcium-complexing substance. Taking into account dilutions which occur during the preparation of fractions containing this substance its concentration may be estimated at about 2.10(-4) in muscle and its calcium stability constant was found to be about 1.5.10(5)M(-1). Preliminary evidence suggests that the calcium-binding substance is a protein.
摘要
一种可溶性马什肌肉松弛制剂的高分子量组分已被证明含有一种钙络合物质。通过研究该组分与螯合树脂100在不同总钙浓度下对钙的竞争性质，可以计算出这种钙络合物质的浓度和钙稳定性常数。考虑到在制备含有该物质的组分过程中发生的稀释情况，其在肌肉中的浓度估计约为2×10⁻⁴，其钙稳定性常数约为1.5×10⁵M⁻¹。初步证据表明，钙结合物质是一种蛋白质。
相似文献
1
Calcium binding by particle-free supernatants of homogenates of skeletal muscle.骨骼肌匀浆无颗粒上清液与钙的结合
J Gen Physiol. 1965 Sep;49(1):131-49.
2
Sarcoplasmic reticulum. VII. Properties of a phosphoprotein intermediate implicated in calcium transport.肌浆网。VII. 与钙转运相关的磷蛋白中间体的特性。
J Biol Chem. 1969 Feb 25;244(4):613-20.
3
Examination of the calcium-modulated protein S100 alpha and its target proteins in adult and developing skeletal muscle.
Cell Motil Cytoskeleton. 1991;20(4):325-37. doi: 10.1002/cm.970200408.
4
The nature of the muscle-relaxing factor. II. Some physicochemical properties.肌肉松弛因子的性质。II. 一些物理化学性质。
J Gen Physiol. 1963 May;46(5):893-904. doi: 10.1085/jgp.46.5.893.
5
The binding of arsenazo III to cell components.偶氮胂III与细胞成分的结合。
Biochim Biophys Acta. 1980 May 7;629(2):317-27. doi: 10.1016/0304-4165(80)90104-x.
6
Biochemical characteristics of free and junctional sarcoplasmic reticulum and of transverse tubules in human skeletal muscle.人类骨骼肌中游离及连接肌浆网和横小管的生化特性
Muscle Nerve. 1989 Apr;12(4):323-31. doi: 10.1002/mus.880120411.
7
Calcium binding activity of microsomal fraction of rabbit rad muscle.
J Biochem. 1968 Mar;63(3):324-31.
8
Interaction of S100A1 with the Ca2+ release channel (ryanodine receptor) of skeletal muscle.S100A1与骨骼肌的Ca2+释放通道（雷诺丁受体）的相互作用。
Biochemistry. 1997 Sep 23;36(38):11496-503. doi: 10.1021/bi970160w.
9
[Caffeine-induced calcium release from relaxing factor in skeletal muscle. II. Studies on rabbit muscle].
Sapporo Igaku Zasshi. 1969 Apr;35(4):227-34.
10
Properties of skeletal muscle phosphorylase-protein complexes.
Acta Biochim Biophys Acad Sci Hung. 1975;10(3):153-9.
引用本文的文献
1
Immunohistochemical localization of vitamin D-dependent calcium-binding protein in duodenum, kidney, uterus and cerebellum of chickens.鸡十二指肠、肾脏、子宫和小脑中维生素D依赖性钙结合蛋白的免疫组织化学定位
Histochemistry. 1981;71(1):99-116. doi: 10.1007/BF00592574.
2
A simple procedure for purifying mammalian duodenal Ca2+-binding proteins on a 100 mg scale and an investigation of the stoichiometry of their high-affinity binding of Ca2+ ions.一种用于在100毫克规模上纯化哺乳动物十二指肠钙结合蛋白的简单方法，以及对其与钙离子高亲和力结合化学计量学的研究。
Biochem J. 1983 Jun 1;211(3):709-16. doi: 10.1042/bj2110709.
3
A hormone dependent calcium-binding protein in the mantle edge of the freshwater snail Lymnaea stagnalis.淡水蜗牛椎实螺外套膜边缘中的一种激素依赖性钙结合蛋白。
Calcif Tissue Int. 1981;33(5):505-8. doi: 10.1007/BF02409481.
4
Comparison of bone and osteosarcoma adenylate cyclase. Effects of Mg2+, Ca2+, ATP4- and HATP3- in the assay mixture.骨与骨肉瘤腺苷酸环化酶的比较。测定混合物中Mg2+、Ca2+、ATP4-和HATP3-的作用。
Biochem J. 1980 Mar 1;185(3):629-37. doi: 10.1042/bj1850629.
5
Protein-bound calcium in tumors.
Naturwissenschaften. 1970 Nov;57(11):547-8. doi: 10.1007/BF00625334.
6
[Calcium-binding protein of human intestinal mucosa in uremia].[尿毒症患者人肠黏膜钙结合蛋白]
Klin Wochenschr. 1972 Jun 15;50(12):603-5. doi: 10.1007/BF01488778.
7
Calcium-activated tension of skinned muscle fibers of the frog. Dependence on magnesium adenosine triphosphate concentration.青蛙皮肤肌肉纤维的钙激活张力。对三磷酸镁腺苷浓度的依赖性。
J Gen Physiol. 1974 Jun;63(6):722-39. doi: 10.1085/jgp.63.6.722.
8
A phosphate-acceptor protein related to parvalbumins in dogfish skeletal muscle.一种与角鲨骨骼肌中微小清蛋白相关的磷酸受体蛋白。
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2198-2202. doi: 10.1073/pnas.71.6.2198.
9
Oxidation states of peroxidase.过氧化物酶的氧化态
Mol Cell Biochem. 1973 Nov 15;2(1):39-52. doi: 10.1007/BF01738677.
10
Effect of human calcitonin on the sarcoplasmic reticulum of the human heart.
Naturwissenschaften. 1973 Jan;60(1):53. doi: 10.1007/BF00591773.
本文引用的文献
1
A NEW PROTEIN COMPONENT PARTICIPATING IN THE SUPERPRECIPITATION OF MYOSIN B.一种参与肌球蛋白B超沉淀反应的新蛋白质成分。
J Biochem. 1964 Jun;55:604-13. doi: 10.1093/oxfordjournals.jbchem.a127933.
2
THE VELOCITY OF CALCIUM BINDING OF ISOLATED SARCOPLASMIC RETICULUM.分离的肌浆网钙结合速度
J Biochem. 1964 Jun;55:599-603. doi: 10.1093/oxfordjournals.jbchem.a127932.
3
RELAXING PARTICLES OF SKELETAL MUSCLE.骨骼肌的舒张颗粒
Fed Proc. 1964 Sep-Oct;23:905-8.
4
ROLE OF CALCIUM IN CONTRACTION AND RELAXATION OF MUSCLE.钙在肌肉收缩和舒张中的作用。
Fed Proc. 1964 Sep-Oct;23:896-900.
5
SARCOPLASMIC RETICULUM. I. THE UPTAKE OF CA++ BY SARCOPLASMIC RETICULUM FRAGMENTS.肌浆网。一、肌浆网片段对钙离子的摄取。
J Biol Chem. 1964 Feb;239:648-58.
6
EFFECTIVENESS OF RELAXING FACTOR IN A CALCIUM BUFFERED SYSTEM.钙缓冲系统中松弛因子的有效性
Life Sci (1962). 1964 Mar;3:287-90. doi: 10.1016/0024-3205(64)90072-4.
7
Properties of the O18 exchange reaction catalyzed by heavy meromyosin.重酶解肌球蛋白催化的O18交换反应的特性
J Biol Chem. 1963 May;238:1708-13.
8
The nature of the muscle-relaxing factor. II. Some physicochemical properties.肌肉松弛因子的性质。II. 一些物理化学性质。
J Gen Physiol. 1963 May;46(5):893-904. doi: 10.1085/jgp.46.5.893.
9
Intracellular localization of the skeletal-muscle relaxing factor.骨骼肌松弛因子的细胞内定位
Biochem J. 1962 May;83(2):364-8. doi: 10.1042/bj0830364.
10
The sarcotubular system of frog skeletal muscle. A morphological and biochemical study.青蛙骨骼肌的肌管系统。一项形态学与生物化学研究。
J Biophys Biochem Cytol. 1961 Aug;10(4)Suppl(4):201-18. doi: 10.1083/jcb.10.4.201.
Zotero 插件