Dogterom A A, Doderer A
Calcif Tissue Int. 1981;33(5):505-8. doi: 10.1007/BF02409481.
By means of gel filtration and ion exchange chromatography on calcium-saturated Chelex-100, a calcium-binding fraction was isolated from the mantle edge of the freshwater snail lymnaea stagnalis. This fraction was not present in other tissues. Treatment with trypsin caused a disappearance of the calcium-binding capacity, proving that the active substance in this fraction is a protein (calcium-binding protein; CaBP). Removal of the growth hormone-producing neuroendocrine light green cells resulted in a strong decrease of the amount of CaBP. It is concluded that L. stagnalis possesses a hormone-dependent CaBP, probably responsible for the maintenance of a high calcium concentration in that part of the mantle that produces the outer crystalline layer of the shell.
通过在钙饱和的Chelex - 100上进行凝胶过滤和离子交换色谱法，从淡水蜗牛椎实螺的外套膜边缘分离出一个钙结合组分。该组分在其他组织中不存在。用胰蛋白酶处理导致钙结合能力消失，证明该组分中的活性物质是一种蛋白质（钙结合蛋白；CaBP）。去除产生生长激素的神经内分泌浅绿色细胞导致CaBP量大幅减少。得出结论，椎实螺拥有一种激素依赖性CaBP，可能负责维持外套膜中产生贝壳外结晶层的那部分的高钙浓度。
