Pridham T G, Hall H H, Jackson R W
Appl Microbiol. 1965 Nov;13(6):1000-4. doi: 10.1128/am.13.6.1000-1004.1965.
The effects of antibiotics, sulfonamides, and other antimicrobial agents on vegetative cultures of five strains of milky disease bacteria were compared with those on Bacillus subtilis Cohn emend. Prazmowski, Staphylococcus aureus Rosenbach, Sarcina lutea Schroeter, Escherichia coli (Migula) Castellani and Chalmers, Saccharomyces pastorianus Hansen, and Mucor ramannianus Moel. Similar numbers of viable cells of each organism were exposed to the test materials by use of an antibiotic-sensitivity disc method adapted from techniques recommended by the Food and Drug Administration in the Federal Register. The results suggest that vancomycin or ristocetin, as well as a few other materials, might be useful in controlling contamination either during culture of the fastidious milky disease bacteria or in large populations of vegetative cells undergoing treatment to induce sporulation. Inhibitory concentrations of vancomycin and ristocetin in shaken-tube tests were much lower than expected in comparison with results of sensitivity-disc tests on the milky disease bacteria. Sublethal concentrations of the two antibiotics elicited some morphological change in the bacteria.
将抗生素、磺胺类药物及其他抗菌剂对五株乳状病细菌营养培养物的作用，与它们对枯草芽孢杆菌（科恩修订，普拉兹莫夫斯基）、金黄色葡萄球菌（罗森巴赫）、藤黄八叠球菌（施勒特尔）、大肠杆菌（米古拉，卡斯泰拉尼和查默斯）、巴斯德毕赤酵母（汉森）和拉曼毛霉（默尔）的作用进行了比较。通过采用食品药品管理局在《联邦公报》中推荐的技术改编的抗生素敏感性圆盘法，使每种生物体数量相似的活细胞接触测试材料。结果表明，万古霉素或利托菌素以及其他一些物质，可能有助于在培养挑剔的乳状病细菌期间或在大量进行诱导孢子形成处理的营养细胞中控制污染。与对乳状病细菌的敏感性圆盘测试结果相比，在摇管试验中万古霉素和利托菌素的抑制浓度远低于预期。这两种抗生素的亚致死浓度引起了细菌的一些形态变化。
