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链霉菌气生菌丝体颜色的比色测定

COLORIMETRIC DETERMINATION OF COLOR OF AERIAL MYCELIUM OF STREPTOMYCETES.

作者信息

LYONS A J, PRIDHAM T G

出版信息

J Bacteriol. 1965 Jan;89(1):159-69. doi: 10.1128/jb.89.1.159-169.1965.

DOI:10.1128/jb.89.1.159-169.1965
PMID:14255657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC315564/
Abstract

Lyons, Allister J., Jr. (Northern Regional Research Laboratory, Peoria, Ill.) and Thomas G. Pridham. Colorimetric determination of color of aerial mycelium of streptomycetes. J. Bacteriol. 89:159-169. 1965.-For some time, streptomycete taxonomists have been seeking to describe more accurately the colors of aerial mycelium. Some of the descriptive systems involve many different color names and groups. Others combine many colors into a few groups. All the systems and methods leave much to be desired. To obtain an accurate description, a colorimeter with a reflectance attachment was used to examine streptomycete aerial mycelium of 37 strains, representing all of the major aerial mycelium color groups. Each color was characterized by three values: dominant wavelength in millimicrons, and purity and brightness in percentages. All colors of aerial mycelium were of low purity (< 25%). Most of the dominant wavelengths were in the yellow to yellow-green bands of the spectrum. Most of the color tabs matched visually with the streptomycete strains had purities of a higher value than those of the cultures. The reflectance instrument seems to allow an objective description, and its use may help to clarify the color problem with streptomycetes. It is concluded that present color descriptions are inadequate and that the significance of color in speciation requires critical examination.

摘要

小阿利斯特·J·莱昂斯(伊利诺伊州皮奥里亚市北方地区研究实验室)和托马斯·G·普里德姆。链霉菌气生菌丝体颜色的比色测定。《细菌学杂志》89:159 - 169。1965年。——一段时间以来,链霉菌分类学家一直在寻求更准确地描述气生菌丝体的颜色。一些描述系统涉及许多不同的颜色名称和类别。另一些则将许多颜色合并为少数几个类别。所有这些系统和方法都有许多不足之处。为了获得准确的描述,使用了一台带有反射附件的比色计来检测37个菌株的链霉菌气生菌丝体,这些菌株代表了所有主要的气生菌丝体颜色类别。每种颜色由三个值来表征:以毫微米为单位的主波长,以及以百分比表示的纯度和亮度。气生菌丝体的所有颜色纯度都很低(<25%)。大多数主波长处于光谱的黄色到黄绿色波段。大多数在视觉上与链霉菌菌株匹配的色卡的纯度值高于培养物的纯度值。反射仪器似乎能够进行客观描述,其使用可能有助于阐明链霉菌的颜色问题。得出的结论是,目前的颜色描述并不充分,而且颜色在物种形成中的意义需要进行批判性研究。

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本文引用的文献

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Proposal to designate strain ATCC 3004 (IMRU 3004) as the neotype strain of Streptomyces albus (Rossi-Doria) Waksman and Henrici.关于指定菌株ATCC 3004(IMRU 3004)为白色链霉菌(罗西 - 多里亚)瓦克斯曼和亨利西新模式菌株的提议。
J Bacteriol. 1962 Feb;83(2):370-80. doi: 10.1128/jb.83.2.370-380.1962.
3
COLOR AND STREPTOMYCETES. REPORT OF AN INTERNATIONAL WORKSHOP ON DETERMINATION OF COLOR OF STREPTOMYCETES.颜色与链霉菌。链霉菌颜色测定国际研讨会报告
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System of color wheels for streptomycete taxonomy.用于链霉菌分类学的色轮系统。
Appl Microbiol. 1963 Jul;11(4):335-8. doi: 10.1128/am.11.4.335-338.1963.
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